油菜蜂花粉中黄酮类化合物的提取工艺研究  

Study on Extraction Technology of Flavonoids from Rape Bee Pollen

作　　者：卢挺 郭雪微[2] 马兰玉 吴玲歌 

机构地区：[1]青海省产品质量监督检验所,青海西宁810008 [2]北京市大兴区人民医院,北京102600

出　　处：《食品科学》2008年第10期148-152,共5页Food Science

基　　金：青海省重点科技攻关项目(2002-N-119);科技部农业科技成果转化资金项目(02EFN216301200)

摘　　要：本研究采用乙醇提出、水沉淀,并结合大孔树脂富集、乙醇洗脱等方法进行破壁油菜蜂花粉中黄酮类化合物的提取工艺研究,获得较好结果。花粉总黄酮的转移率可达70%以上,提取物的黄酮类化合物含量大于50%。采用柱式分离、有机溶剂分步洗脱等方法从提取物中分离出芦丁、槲皮素、山奈酚、异鼠李素等四种单体黄酮,经核磁共振结构鉴定证实的有芦丁、槲皮素、山奈酚等三种。同时,采用富集、洗脱等方法分离出β-谷甾醇、β-豆甾醇等两种生物活性物质并经核磁共振结构鉴定证实。Technological extraction of flavonoids in rape bee pollen was studied by using the methods of ethyl alcohol extraction and water precipitation combined with macroporous resin concentration and ethyl alcohol elution, and the satisfying results were obtorined. The transfer rate of flavonoids in rape bee pollen is over 70%, and the content of flavonoids is over 50%. Rutin, quercetin, kaempferol and isorhamnetin are separated from extracts by the methods of column separation and stepping elution of organic solvent. Rutin, quercetin and kaempferol are verified by appraisal of nuclear magnetic resonance. Meanwhile, β - sitosterol and β -stigmasterol are separated by the methods of concentration and elution. The bioactive substances are verified by nuclear magnetic resonance.

关 键 词：蜂花粉 黄酮类化合物 提取 

分 类 号：O623.54[理学—有机化学]