猪弓形虫病PCR诊断方法的建立  

Development of PCR Assay for Diagnoses of Swine Toxoplasmosis

作　　者：王海燕[1] 马广鹏[2] 任庆娥[1] 姚雷[1] 宁长申[1] 张龙现[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]科技部中国农村技术开发中心,北京100862

出　　处：《河南农业大学学报》2008年第5期524-527,共4页Journal of Henan Agricultural University

基　　金：河南省高校创新人才培养工程(豫教高[2004]294号);河南农业大学2007年创新基金项目

摘　　要：以具有35个拷贝的重复序列B1基因作为弓形虫种特异的遗传标记,建立诊断弓形虫病特异性的PCR方法.敏感性和特异性试验结果表明:该PCR方法最低能检测到10个弓形虫速殖子DNA,且与寄生于猪体内的6种常见微生物无交叉反应,说明该PCR方法具有高度的敏感性和特异性.动物感染试验检测结果表明,在试验动物出现明显临床症状时,对取材的7个组织样品用该PCR方法检测,有6个组织样品为阳性,其中扩增结果最清晰的的组织为肺和肝组织.PCR was developed to detect the pathogenic protozoan Toxoplasma gondii using species-spe- cific primers based on identification of a 35-fold-repetitive gene （the B1 gene）. Results of experment on Sensitivity and specificity show that the assay was able to detect at least 10 tachyzoites of T. gondii. And no cross reaction was detected using this assay from DNAs of a variety of 6 other organisms, which were often found in swines. Experimental infection showed the specific PCR products of T. gondii could be amplified from six of the seven examined tissues of mice. Both the lung and the liver tissues appeared the clearest PCR amplification fragments.

关 键 词：弓形虫 猪 B1基因 PCR 

分 类 号：S852.7[农业科学—基础兽医学]