不同基因载体转导人骨髓间充质干细胞比较  

Comparison of Transduction Efficiencies of Various Gene Vectors in Human Bone-marrow-derived Mesenchymal Stem Cells

作　　者：刘正山[1,2] 张成[1,2] 尚延昌[3] 熊符[4] 冯善伟[2] 李勇[2] 许勇峰[1] 周畅[1] 

机构地区：[1]中山大学第一附属医院神经内科,广州510080 [2]中山大学干细胞与组织工程研究中心,广州510080 [3]中国人民解放军总医院老年神经内科,北京100853 [4]南方医科大学遗传学教研室,广州510515

出　　处：《中国医学科学院学报》2008年第5期569-573,I0003,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30170337,30870851)~~

摘　　要：目的比较腺病毒载体、腺相关病毒载体、杆状病毒载体和质粒载体对人骨髓间充质干细胞(hBMSCs)的转导效率。方法体外原代培养hBMSCs,进行腺病毒载体、腺相关病毒载体、杆状病毒载体和质粒载体转染实验。采用倒置荧光显微镜及流式细胞仪检测经基因载体转染后的hBMSCs胞内目的蛋白表达情况。结果倒置荧光显微镜观察结果显示,基因载体转染后的部分hBMSCs胞内有GFP表达而发绿色荧光,其中杆状病毒转导的hBMSCs荧光强度较强。流式细胞仪检测结果表明,腺病毒载体、腺相关病毒载体及质粒载体的转导效率和绿色荧光蛋白阳性细胞平均荧光强度分别是42%、37%、22%和158、115、77,均明显低于杆状病毒的70%(P<0.01)和212(P<0.05)。结论杆状病毒载体对hBMSCs的转导效率高于腺病毒载体、腺相关病毒载体和质粒载体,有望成为人体基因治疗研究中更为理想的基因载体。Objective To compare the transduction efficiencies of adenoviral vector, adeno-associated viral vector, baculoviral vector, and plasmid vector in human bone-marrow-derived mesenchymal stem cells （hBMSCs）. Methods The hBMSCs were cultured in vitro and transducted with the adenoviral vector, adeno- associated viral vector, baculoviral vector, and plasmid vector. The expression of target protein was observed by inverted fluorescent microscopy and flow cytometry. Results Inverted fluorescent microscopy showed that some of the hBMSCs after transduction expressed the green fluorescent protein （GFP） and the hBMSCs transdueted with baculoviral vector expressed more GFP than those of other three vectors. Flow cytometry showed that the transduction efficiencies and mean fluorescence intensities of the adenoviral vector, adeno-associated viral vector, and plasmid vector were 42%, 37%, and 22% and 158, 115, and 77, respectively, which were significantly lower than those of baculoviral vector （70% , P 〈 0.01 ; 212, P 〈 0.05 ; respectively）. Conclusion Compared with the adenoviral vector, adeno-associated viral vector, and plasmid vector, the baculoviral vector has higher transduction efficiency in hBMSCs and therefore may be a more suitable gene vector for research in human gene therapy.

关 键 词：杆状病毒 基因载体 转导 人骨髓间充质干细胞 

分 类 号：R373.9[医药卫生—病原生物学]