hPOT1 RNA干扰对人胃癌细胞株BGC-823端粒长度和hTERT表达的影响  

Alterations of Telomere Length and hTERT Expression in Human Gastric Cancer Cell Line BGC-823 with Silencing of hPOT1 by RNA Interference

作　　者：宁晓燕[1] 房殿春[1] 贴君[1] 程永波[1] 张汝钢[1] 王洪斌[1] 杨柳芹[1] 

机构地区：[1]第三军医大学西南医院全军消化病研究所,400038

出　　处：《胃肠病学》2008年第10期607-610,共4页Chinese Journal of Gastroenterology

基　　金：国家自然科学基金资助项目(30470796)

摘　　要：背景:人端粒保护蛋白1(hPOT1)的主要作用为保护端粒和调节端粒长度。目的:探讨hPOT1在人胃癌细胞中对端粒长度的调节作用及其可能机制。方法:以RNA干扰(RNAi)技术抑制人胃癌细胞株BGC-823中hPOT1的表达,以实时荧光定量聚合酶链反应(PCR)检测细胞端粒长度,以半定量逆转录聚合酶链反应(RT-PCR)检测人端粒酶逆转录酶(hTERT)mRNA表达。结果:以RNAi技术抑制hPOT1表达后,BGC-823细胞端粒长度明显缩短,反映端粒相对长度的T/S值显著小于亲本BGC-823细胞组和阴性对照组(1.383±0.091对2.758±0.647和3.043±0.548,P<0.05),亲本细胞组与阴性对照组之间则无明显差异。hPOT1 RNAi组细胞hTERT mRNA表达亦明显下调。结论:在人胃癌细胞中,hPOT1能正性调节端粒长度;在hPOT1下调引起端粒缩短的环节中,hTERT表达下降可能起一定作用。Protection of telomere and regulation of telomere length are the two main functions of human protection of telomeres 1 （hPOT1） protein. Aims： To investigate the effect of hPOT1 on regulation of telomere length in human gastric cancer ceils and its potential mechanism. Methods： Expression of hPOT1 in human gastric cancer cell line BGC-823 was inhibited by RNA interference （RNAi）. Telomere length of BGC-823 cells was measured by real-time fluorescent quantitative polymerase chain reaction （PCR）, and expression of human telomerase reverse transcriptase （hTERT） mRNA was determined by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR）. Results： Telomere length of BGC-823 cells in hPOT1 RNAi group was significantly shorter than that of parent BGC-823 cells and negative controls （P〈0.05）, whereas no significant difference was observed between parent cells and negative controls. T/S ratios （reflecting relative telomere length） in hPOT1 RNAi group, parent BGC-823 cells group and negative control group were 1.383±0.091, 2.758±0.647 and 3.043±0.548, respectively. Expression of hTERT mRNA was also down-regulated significantly in hPOT1 RNAi group. Conclusions： hPOT1 regulates the telomere length positively in human gastric cancer cells. Decrease of hTERT expression may be involved in the telomere shortening induced by hPOT1 down-regulation.

关 键 词：端粒保护蛋白1 RNA 小分子干扰 端粒长度 端粒酶逆转录酶 细胞系 肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]