几丁糖顺铂缓释药膜的制备及体外抑制人胃腺癌SGC-7901细胞生长的研究  

Preparation of Cisplatin Chitosan Slow-release Film and in Vitro its Inhibition Gastric Gancer Cell Growth

作　　者：谢小平[1] 魏玉亮[1] 朱共元[1] 陈文学 揭志刚[1] 

机构地区：[1]南昌大学第一附属医院普外二科,330006 [2]江西省肿瘤研究所,330029

出　　处：《实用癌症杂志》2008年第6期551-553,共3页The Practical Journal of Cancer

基　　金：江西省卫生厅资助项目(编号040326)

摘　　要：目的观察几丁糖-顺铂缓释药膜体外缓释性和对人胃腺癌SGC-7901细胞生长的抑制作用。方法将几丁糖与顺铂混合,加入交联剂戊二醛,真空干燥,制成几丁糖-顺铂缓释药膜,检测药膜的自然降解性、药膜顺铂含量和药物包封率,同时观察药膜体外缓释顺铂及其对胃癌细胞生长的抑制作用。结果几丁糖-顺铂缓释药膜在含溶菌酶的生理盐水中于8天后开始自然降解,60天后降解率达83.33%;药膜载药率为(7.30±0.20)%,药物包封率为(51.78±2.10)%;药膜体外释放顺铂量于第1天达75.40μg/ml,第2天释放量明显降低,为21.35μg/ml,以后呈较低水平缓慢释放,第7天达3.94μg/ml,并且,其浸出液体外抑制胃癌细胞生长的抑制率第1天为84.24%,以后渐降低,第7天达29.81%。结论几丁糖-顺铂缓释药膜在体外可较好地缓释顺铂,对人胃腺癌SGC--7901细胞的生长有较持久地抑制作用。Objective The aim of this study was to observe the characteristics of chitosan cisplatin slow-release film for release cisplatin and inhibiting gastric cancer cell growth in vitro.Methods Chitosan cisplatin slow-release film was prepared by mixing chitosan with cisplatin,and joining cross-linking agent glutaraldehyde in vacuum freeze dryer.After natural degradation,the platinum content,cisplatin encapsulation efficiency of the film and the cisplatin release rate were detected.In vitro inhibition of gastric cancer cell growth was then studied.Results On day 8,Chitosan cisplatin slow-release film were degraded naturally in saline solution containing lysozyme,and on day 60,weight lost rate of the film was 83.33%.Cisplatin content cisplatin was（7.30±0.20）%,and cisplatin encapsulation efficiency was（51.78±2.10）%.The concentrations of cisplatin releasing from the film in vitro was 75.40μg/ml on the 1st day,21.35μg/ml on the 2nd day,and 3.94 μg/ml on the 7th day.The inhibition ratio for gastric cancer cell growth in vitro with the leachate of the film was 84.24% on day 1,29.81% on day 7.Conclusion Chitosan cisplatin slow-release film had adequate for release cisplatin slowly in vitro,and had depressant effect lastingly for gastric adenocarcinoma SGC-7901 cell growth.

关 键 词：几丁糖膜 顺铂 缓释性 胃癌细胞 

分 类 号：R735.2[医药卫生—肿瘤]