高效阴离子交换色谱-脉冲安培检测法测定牛乳及制品中的唾液酸  

Determination of Sialic Acid in Milk and Products Using High Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection

作　　者：唐坤甜[1] 梁立娜[1] 蔡亚岐[1] 牟世芬[1] 

机构地区：[1]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《分析化学》2008年第11期1535-1538,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.200577058);国家重点基础研究计划(973项目)(No.2003CB415001)

摘　　要：建立了高效阴离子交换色谱分离-脉冲安培检测法检测常见唾液酸N-乙酰神经氨酸（Neu5AC）和N-羟乙酰神经氨酸（Neu5Gc）的方法,对影响分离和检测的条件进行了优化。以NaOH和NaAc为淋洗液,在CarboPac PA20阴离子交换柱上梯度分离,在30℃柱温,0.5 mL/min的流速下,20 min内可完成两种唾液酸的分析。使用脉冲安培检测法测定Neu5AC和Neu5Gc的线性范围为5-500μg/L;检出限为3.0和1.8μg/L（25.0μL进样,以3倍信噪比计算检出限）。将该方法用于15个牛乳及制品中Neu5AC和Neu5Gc的测定,两种唾液酸的标准加入回收率为88%-115%。方法具有灵敏、简便和环境友好的特点,样品前处理方法简单,方法适用性好。A resolved succinct environmental-friendly chromatographic method was developed for the determination of N-acetylncuraminic acid （Neu5AC） and N-glycolylneuraminic acid （Neu5Gc） in bovine milk and products using high-performance anion exchange chromatography coupled with pulsed amperometric detection. The conditions affecting the separation, detection and sialie acid release from milk were optimized. The separation was carried out on CarboPac PA20 anion-exchange column using 100 mmol/L NaOH, 20 -300 mmol/L NaAc gradient eluting in 20 min at the flow rate of 0.5 mL/min. The separation and detection temperature were both at 30 ℃. The detection was carried out on a gold working electrode. The detection limits were 3.0 and 1.8 μg/L and the linear range of 5 to 500 μg/L was obtained （25.0 μL injection, three times of the baseline noise）. The spiked recovery of NeuSAC and NeuSGc in milk was 88% -115%.

关 键 词：高效阴离子交换色谱 脉冲安培检测 唾液酸 牛乳 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]