毛细管电泳柱后衍生激光诱导荧光测定动物组织中卡那霉素类抗生素  

Capillary Zone Electrophoresis Analysis for Kanamycins in Animal Tissue with Post-column Derivatization and Laser-induced Fluorescence

作　　者：余长柱[1] 何友昭[1] 李连[1] 韩芳[1] 淦五二[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026

出　　处：《分析化学》2008年第11期1551-1554,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.20675075;20275035)资助项目

摘　　要：采用毛细管电泳柱后衍生激光诱导荧光测定3种卡那霉素类抗生素。研究了在反向电渗流条件下HAc-NaAc缓冲液酸度和浓度对卡那霉素类抗生素分离的影响,研究了Na2B4O7缓冲体系和柱后衍生试剂萘二醛/2-巯基乙醇浓度对检测信号的影响。采用50 mmol/L HAc-NaAc（pH5.0）＋0.5 mmol/L CTMAB溶液为分离缓冲液,样品在-15 kV下分离,与柱后衍生试剂1.0 mmol/L NDA＋8.0 mmol/L 2-ME＋35 mmol/LNa2B4O7（pH10.0）的30%（V/V）甲醇溶液反应,激发诱导荧光检测卡那霉素类抗生素检出限为3.6×10^-5-5.2×10^-5g/L;本方法用于动物组织中卡那霉素类抗生素残留检测,相对标准偏差小于7.2%;回收率为90.2%-95.3%（n=4）。A selective and sensitive method for the determination of kanamycins with post-column derivatization in capillary zone electrophoresis and laser induced fluorescence detection was proposed. The derivatization reaction was carried out using a coaxial-gap reactor with a derivatization reagent of naphthalene-2,3-dicarboxaldehyde（NDA）. The labeled kanamycins were detected with the excitation wavelength at 473 nm in laser induced fluorescence. The separation was carried out at - 15 kV with reversed electroosmotie flow in 50 mmol/L sodium acetic buffer （pH 5.0） containing 0.5 mmol/L cetyhrimethyl ammonium. The derivatization was performed with the derivatization reagent of 1.0 mmol/L thalene-2,3-dicarboxaldehyde, 8.0 mmol/L 2-merc-aptoethanol and 35 mmol/L soldium （pH 10.0） in 30% （V/V） methanol tetraborate buffer. The limits of detection for kanamycins ranging from 3.6 ×10^-5 to 5.2 ×10^-5 g/L and the separation efficiencies from 2.0 ×10^-5to 2.4 ×10^-5 theoretical plates were obtained. The method has been employed to analyze kanamycin residues in animal tissues.

关 键 词：毛细管区带电泳 激光诱导荧光 柱后衍生 卡那霉素类抗生素 

分 类 号：TS207.53[轻工技术与工程—食品科学]