喉鳞状细胞癌中微血管密度与COX-2、VEGF表达及临床意义  

Angiogenesis and expression of Cox-2,VEGF in laryngeal squamous cell carcinoma

作　　者：李正贤[1] 刘庚勋[2] 颜美荣[1] 刘阳云[1] 

机构地区：[1]解放军第一六三医院耳鼻咽喉科 [2]解放军第一六三医院病理科,湖南长沙410003

出　　处：《中国耳鼻咽喉颅底外科杂志》2008年第5期339-343,共5页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的探讨COX-2、VEGF在喉鳞状细胞癌发生发展及侵袭转移中的作用。方法应用免疫组化Elivision法检测30例喉鳞状细胞癌、10例声带非典型增生中COX-2、VEGF的表达,并在显微镜下观察微血管密度(MVD)。结果VEGF在30例喉鳞状细胞癌和10例声带非典型增生中的阳性表达率分别为86.7%,20%;COX-2在两组中的阳性表达率分别为76.7%、10%;喉鳞状细胞癌及声带非典型增生微血管密度分别为65.85±13.06、15.37±3.47。COX-2、VEGF阳性表达和微血管密度两组间差异有统计学意义。结论COX-2、VEGF在喉鳞状细胞癌中的高表达,提示有新生血管化的发生,可能与喉鳞状细胞癌的发生、发展及侵袭转移有关。选择性COX-2、VEGF抑制剂可望成为基因治疗喉鳞状细胞癌新的靶点。Objective To investigate the role of eyclooxygenases-2 （ COX-2 ） and vascular endothelial growth factor （VEGF） in the occurrence, development, and matastasis of laryngeal squamous cell carcinoma. Methods The immunohistochemical Elivision method was used for detecting the expression of COX- 2 and VEGF in 3 0 specimens of laryngeal squamous cell carcinoma and 1 0 specimens of vocal cord atypical hyperplasia , their mierovaseular density （ MVD ） was measured as well. Results The positive rates of COX - 2 were 7 6.7 % and 10 % in laryngeal squamous cell carcinoma group and vocal cord atypical hyperplasia group respectively, while those of VEGF were 86. 7% and 20 %. The differences of positive rates of COX-2, VEGF, and MVD between the two groups were statistically significant. Conclusion The high expressions of COX-2 and VEGF indicate abnormal angiogenesis in laryngeal squamous cell carcinoma, which suggests that COX-2 and VEGF play an important role in the incidence, development, and the metastasis of laryngeal squamous cell carcinoma and that specific COX-2 and VEGF inhibitors may be new targets for the gene-therapy.

关 键 词：环氧化酶2 血管内皮生长因子 喉肿瘤 癌 鳞状细胞 血管形成 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤] R392.112[医药卫生—临床医学]