HBV转染增强细胞因子上调PD-L表达  

HBV transinfected enhances the PD-L expression of cytokines stimulating

作　　者：吴学杰[1] 陈姬[1] 朱曼华[1] 洪源[1] 钟璐 王贵强[1] 

机构地区：[1]北京大学第一医院感染疾病科,100034 [2]辽宁省阜新市传染病医院

出　　处：《中华实验和临床病毒学杂志》2008年第5期333-335,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：卫生部临床学科重点项目（20010911）;国家自然科学基金资助项目（No.30771905）

摘　　要：目的研究HBV转染的肝细胞系（HepG2．2．15细胞）在细胞因子作用下能否上调PD-L表达。方法应用肝细胞系（HepG2细胞和HepG2．2．15细胞）为模型，用IL-4、IFN-α、IFN-γ（终质量浓度均为10ng／ml，刺激时间为12h）作用于上述细胞系，采用RT-PCR技术检测细胞因子作用前后PD-L表达情况。结果无论是否转染HBV，IFN-α和IFN-γ均能诱导肝细胞系（HepG2细胞和HepG2．2．15细胞）PD-L1表达；而IL-4不能诱导PD-L1表达，IL-4、IFN-α、IFN-γ均能诱导转染了HBV的HepG2．2．15细胞PD-L2表达，仅IFN-γ能诱导未转染HBV的HepG2细胞PD-L2表达。结论IFN-α和IFN-γ有较强诱导肝细胞系（HepG2细胞和HepG2．2．15细胞）PD-L1表达上调的作用，HBV在细胞因子诱导HepG2．2．15细胞PD-L2表达中具有促进作用。Objective To investigate whether the PD-L expression in the liver cell lines transinfected with HBV（HepG2.2.15 cells） can be up-regulated after cytokines stimulating. Methods To apply the liver cell lines （HepG2 cells and HepG2.2.15 cells） as a model, the cells were stimulated with IL-4, IFN-α and IFN-γ （final concentration were 10 ng/ml, stimulated for 12 hours） and RT-PCR was carried out to determine the PD-L expression before and after eytokines stimulating. Results Whether or not transinfected with HBV, IFN-α and IFN-γ both can induce the liver cell lines （HepG2 cells and HepG2.2.15 cells） PD-L1 expression while IL-4 can not; IL-4, IFN-α, IFN-γ all can induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, only IFN-γ can induce the PD-L2 expression in HepG2 cells which was not transinfected with HBV. Conclusion IFN-α, IFN-γ both can induce the PD-L1 expression in HepG2 cells and HepG2.2.15 cells, while it is easy for cytokines to induce the PD-L2 expression in HepG2.2.15 cells which was transinfected with HBV, this may provide a potential mechanism of the molecular basis for chronic HBV infection.

关 键 词：细胞因子类 肝炎病毒 乙型 PD-L1 PD-L2 

分 类 号：R512.62[医药卫生—内科学]