阿卡波糖产生菌的选育和发酵工艺优化  

Breeding of Acarbose-producing Strain and Improvement of Fermentation Technology

作　　者：张琴[1] 胡海峰[1] 朱宝泉[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》2008年第11期820-822,共3页Chinese Journal of Pharmaceuticals

基　　金：上海市重点项目资助(064319031)

摘　　要：阿卡波糖产生菌经紫外、NTG、DES和亚硝酸等诱变,筛得杂质组分降低的高产突变株SIPI-AK,发酵水平提高35%;优化高产突变株的罐上补料工艺,阿卡波糖的发酵单位达3130μg/ml,杂质C组分的生成受到控制。An acarbose high-producing mutant SIPI-AK was selected by the treatment of ultraviolet radiation, NTG, DES and HNO2 methods. The acarbose production of this mutant was increased by 35% over the original strain and some impurity components were reduced in its fermentation broth. By optimization of the feeding process, the acarbose titer reached 3130μg/ml and production of the impurity component C was controlled.

关 键 词：阿卡波糖 菌株选育 补料 

分 类 号：Q93[生物学—微生物学]