秋福红树莓叶片离体再生植株研究  

In vitro adventitious shoot regeneration from leaves of red raspberry cul-tivar Autumn Bliss

作　　者：李媛媛[1] 郭修武[1] 代汉萍[1] 刘海涛[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161

出　　处：《果树学报》2008年第6期868-871,F0003,共5页Journal of Fruit Science

基　　金：农业部科技部行业科研专项(nykyzx07-028);农业部"十一五"948项目G-25

摘　　要：以红树莓品种秋福(Rubus L.cv.Autumn Bliss)离体叶片为外植体,研究适合其再生植株的叶片部位、放置方式、植物生长调节剂以及暗培养时间等条件。结果表明,叶片外植体接种于MS+TDZ2.00mg/L+IAA0.10mg/L的培养基,暗培养2～3周转移至正常光照下培养效果最好,愈伤组织形成率、不定芽再生率和外植体不定芽数分别为100.00%、95.83%和(5.57±0.27)个。将再生芽接种于1/2MS+IBA0.10mg/L的培养基中,35d后生根率达100.00%。再生植株炼苗后移栽,30d时成活率达97.30%,成功地建立了红树莓叶片的离体再生体系。Using tissue culture technique, the conditions for adventitious shoot regeneration of leaf explants collected from red raspberry cultivar Autumn Bliss were studied. Results showed that the whole leaf inoculated on MS medium containing 2.00 mg/L TDZ and 0.10 mg/L IAA, dark incubated for the first 2 to 3 weeks and then transferred to light was the most optimum for shoot regeneration, and the callus percentage, the shoot percentage and average shoots number per explants were 100.00%, 95.83% and （5.57±0.27）, respectively. Regenerated shoots were cut and transferred to 1/2 MS medium contain- ing 0.10 mg/L IBA for rooting. After cultured for 35 d, the rooting percentage reached up to 100.00%. The regenerated plants were transplanted in pots where 97.30% plants survived after 30 d. The plant regeneration system from leaf explants of red raspberry was developed successfully.

关 键 词：红树莓 秋福 叶片 离体培养 再生植株 

分 类 号：S663.2[农业科学—果树学]