4个绵羊品种Leptin基因部分片段的SNPs分析  

SNPs analysis on the Leptin gene in four breeds of sheep

作　　者：黄丹丽[1] 陈仁金[1] 杨章平[1] 毛永江[1] 李云龙[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《畜牧与兽医》2008年第8期18-22,共5页Animal Husbandry & Veterinary Medicine

基　　金：江苏省自然科学基金(BK2003040)

摘　　要：利用PCR-SSCP技术对萨福克、道塞特、特克塞尔及滩羊4个绵羊品种358个个体Leptin基因2、3外显子进行多态性分析,共检测到7个SNPs,其中新发现5个SNPs。测序结果表明,在外显子2上无突变。内含子2上存在99位碱基由A突变为G;115位碱基由G突变为A;150位碱基由C突变为T;171位碱基由C突变为T。外显子3上,存在271位碱基由G突变为A,使编码的Arg转变为Gln;316位碱基由C突变为A,使编码的Pro转变为Gln;387位碱基由G突变为T,使编码的Val转变为Leu。PCR-SSCP was applied to detect single nucleotide polymorphism （SNP） of exon2 and exon3 of the leptin gene for 358 sheep, including Poll Dorsets （PD）, Suffolks （SF）, Texels （TX）, and Tan sheep （Tan）. The polymorphisms were demonstrated, and cxon2 and exon3 DNA sequences were got. Seven SNPs were detected, five of which were new SNPs. There was no SNP in exon2, but four SNPs detected in parts of intron2. They were A99G, C150T , Gll5A and C171T. Three SNPs occurred in exon3, all of which resulted in amino acid changes. The G-A polymorphism （271-SNP） was found in an amino acid substitution, from Arg to Gln. The C-A transition （316-SNP） causes an amino acid substitution from Pro to Gln. The G to T substitution （387-SNP） resulted in a Val to Leu amino acid change.

关 键 词：绵羊 LEPTIN GENE SNPS 

分 类 号：S826[农业科学—畜牧学]