微生物杀灭效果试验中的游离氯转化研究  

Reaction of Free Chlorine Transforms into Chloramines in Microorganism Inactivation Experiment

作　　者：刘静[1] 陈超[1] 张晓健[1] 张车琼[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084

出　　处：《环境科学》2008年第11期3054-3058,共5页Environmental Science

基　　金：国家自然科学基金项目(50708050);国家高技术研究发展计划(863)项目(2006AA06Z301)

摘　　要：在实验室研究游离氯灭活微生物的试验中发现,由于试验体系中有机氮的存在,投加游离氯消毒后,游离氯会迅速与之反应转化为无消毒作用的有机氯胺.在对大肠杆菌、铁细菌、铜绿假单胞菌的研究中均发现同样的问题,投加2 mg.L-1的游离氯消毒剂对初始菌浓度为108CFU.mL-1的大肠杆菌进行灭活试验,反应5 min后游离氯就已经为0,而一氯胺和二氯胺的量分别为0.92 mg.L-1和0.4 mg.L-1.为了降低有机氮的干扰,采用多次离心纯化菌液、膜过滤纯化菌液和增加受试菌液的稀释倍数的方法进行测试,发现当菌液浓度降至105CFU.mL-1以下时,游离氯转氯胺的比例可以明显降低;而2种纯化过程并不能分离菌液中的有机氮,进而改善试验中出现的游离氯转氯胺的现象.该结论也说明参加反应的有机氮应来源于细菌自身的细胞物质,有机氮的干扰在消毒的相关研究中需要引起关注.Due to organic N exiting in the pure bacterial cultures, when using free chorine to inactivate microorganisms, freest chlorine transforms into organic N-chloramines which are no use in disinfection process. This reaction happens in E. coli, Pseudomonas aeruginosa inactivation experiments. Added 2 mg·L^-1 free chlorine to inactivate E. coli of 10s CFU·mL^-1, free chlorine exhausts in 5 min while chloramines and dichloramines become 0.92 mg·L^-1and 0.4 mg·L^-1 E. coli is used as test microorganism in following inactivation experiments. E. coli suspension is purified by centrifugal and membrane filtration, but the purification can＇t help to separate the organic N from E. coli suspension, which indicating that the source of oaganic N may be from bacterium cells. Dilution is worked when E. coll concentrations are diluted under 10^5 CFU/mL. This result should be regarded in related researches in future.

关 键 词：消毒 游离氯 大肠杆菌 有机氯胺 

分 类 号：X172[环境科学与工程—环境科学]