进水浓度或HRT变化时微好氧处理微生态机制研究  

Micro-ecology Mechanism on Microaerobic Treatment Performance at Various Influent COD Concentrations or HRTs

作　　者：韩蕙[1] 王晓丹[2] 张燕燕[1] 翟振华[2] 郑少奎[1] 李艳红[2] 

机构地区：[1]北京师范大学环境学院,水环境模拟国家重点实验室,北京100875 [2]首都师范大学生命科学学院,北京100037

出　　处：《环境科学》2008年第11期3087-3092,共6页Environmental Science

基　　金：国家重点基础研究发展规划(973)项目(2006CB403306)

摘　　要：考察改变进水COD浓度(11000、15000、30000 mg/L)或水力停留时间(HRT为42 h、25 h)对模拟高浓度有机废水微好氧连续小试处理效果的影响,并采用荧光原位杂交-流式细胞术(FISH-FCM)、聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)等分子生物学技术及Biolog FF微孔板法分析了不同处理阶段稳定期曝气柱污泥的微生态变化,以探讨进水COD浓度或HRT改变时微好氧处理效果变化的微生态机制.结果表明,在全部4个处理阶段,曝气柱污泥中酵母含量均保持在>99.9%的水平.随着进水COD浓度的上升,污泥浓度由2.0 g/L上升到7.3 g/L,COD比去除速度由2.3 kg/(kg.d)下降到1.7 kg/(kg.d),曝气柱污泥真菌群落结构多样性指数由2.05上升至2.19,代谢多样性指数由4.42上升至4.45;而随着HRT下降,污泥浓度从7.3 g/L下降至6.0 g/L,COD比去除速度从1.7 kg/(kg.d)上升至2.8 kg/(kg.d),真菌群落结构多样性指数和代谢多样性指数则分别从2.19和4.45降至0.79和4.36.作为提高进水有机负荷的主要措施,提高进水COD浓度和缩短HRT对于废水处理效果和曝气柱微生态存在截然相反的影响.A lab-scale continuous experiment was designed to investigate the effect of influent COD concentrations （11 000, 15 000, 30 000 mg/L） or hydraulic retention time （HRT 42, 25 h）on the microareobic treatment performance of simulated high-strength organic wastewater. The molecular microbiological technologies, including Fluorescent in-situ hybridization-Flow Cytometry （FISH-FCM）, Polymerase Chain Reaction-Denaturing Gradient GeI Electrophoresis （PCR-DGGE）, and Biolog - FF assay method, were used to detect the variation of microbial community in the aerated column during the four pseudo-steady-state periods. The yeast contents remained 〉 99.9% throughout the overall experimental periods according to FISH-FCM. Increasing influent COD concentration brought on a rising MLSS （2.0-7.3 g/L） and a reduced specific COD removal rate [ 2.3 - 1.7 kg/（ kg·d） ], structural （ PCR- DGGE）/metabolic （ Biolog FF ） diversity index values of fungal community in the aerated column had an increase of （2.05-2.19）/（4.42-4.45）. Shortening HRT brought on a reduced MLSS （7.3-6.0 g/L） and a rising specific COD removal rate [ 1.7-2.8 kg/（ kg· d）], structural （PCR-DGGE）/metabolie （Biolog FF） diversity index values of fungal community had a decrease of （2.19-0.79）/（4.45-4.36）. Increasing influent COD concentration or shortening HRT has an absolutely adverse effect on the microaerobic treatment performance and micro-ecology in the aeration column although either of them can increase infiuent COD loading up to a higher level.

关 键 词：微好氧 FISH-FCM PCR-DGGE BIOLOG 高浓度有机废水 HRT 微生态 

分 类 号：X703.1[环境科学与工程—环境工程]