Mitochondrial variants may influence the phenotypic manifestation of Leber's hereditary optic neuropathy-associated ND4 G11778A mutation  

作　　者：Wanshi Cai Qun Fu Xiangtian Zhou Jia Qu Yi Tong Min-Xin Guan 

机构地区：[1]Zhejiang Provincial Key Laboratory of Medical Genetics, School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China [2]School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou 325003, China [3]The Third Affiliated Hospital, Xinxiang Medical College, Xinxiang 453003, China [4]The FirstAffiliated Hospital, Fujian Medical University, Fuzhou 350005, China [5]Division of Human Genetics, Cincinnati Children＇s Hospital Medical Center, Cincinnati, Ohio 45229, USA

出　　处：《Journal of Genetics and Genomics》2008年第11期649-655,共7页遗传学报（英文版）

基　　金：Zhejiang Provincial Natural Science Foundation（ZB0202）；Chinese Young Scholar Award（No.30628013）；the National Science Foundation of China to M.X.G and the Key Research and Development Program from Zhejiang Province（No.2004C14005）to J.Q.

摘　　要：We report here the characterization of a five-generation Han Chinese family with Leber＇s hereditary optic neuropathy （LHON）. Strik- ingly, this Chinese family displayed high penetrance and expressivity of visual loss. The average age-of-onset of vision loss was 18 years in this family. Nineteen （11 males/8 females） of 29 matrilineal relatives in this family developed visual loss with a wide range of severity, ranging from blindness to normal vision. Sequence analysis of mitochondrial genome in this pedigree revealed the presence of the ND4 G 11778A mutation and 44 other variants belonging to Asian haplogroup M7b. The G 11778A mutation is present at homoplasmy in matri- lineal relatives of this Chinese family. Of other variants, the C01 G6480A, ND5 T12811C and Cytb A15395G located at highly conserved residues of corresponding polypeptides. In fact, these variants were implicated to be involved in other clinical abnormalities. Here, these variants may act in synergy with the primary LHON-associated Gl1778A mutation. Thus, the mitochondrial dysfunction caused by the primary ND4 G11778A mutation may be worsened by these mitochondrial variants. The results imply that the G6480A, T12811C and A15395G variants might have a potential modifier role in increasing the penetrance and expressivity of the primary LHON-associated G11778A mutation in this Chinese family.We report here the characterization of a five-generation Han Chinese family with Leber＇s hereditary optic neuropathy （LHON）. Strik- ingly, this Chinese family displayed high penetrance and expressivity of visual loss. The average age-of-onset of vision loss was 18 years in this family. Nineteen （11 males/8 females） of 29 matrilineal relatives in this family developed visual loss with a wide range of severity, ranging from blindness to normal vision. Sequence analysis of mitochondrial genome in this pedigree revealed the presence of the ND4 G 11778A mutation and 44 other variants belonging to Asian haplogroup M7b. The G 11778A mutation is present at homoplasmy in matri- lineal relatives of this Chinese family. Of other variants, the C01 G6480A, ND5 T12811C and Cytb A15395G located at highly conserved residues of corresponding polypeptides. In fact, these variants were implicated to be involved in other clinical abnormalities. Here, these variants may act in synergy with the primary LHON-associated Gl1778A mutation. Thus, the mitochondrial dysfunction caused by the primary ND4 G11778A mutation may be worsened by these mitochondrial variants. The results imply that the G6480A, T12811C and A15395G variants might have a potential modifier role in increasing the penetrance and expressivity of the primary LHON-associated G11778A mutation in this Chinese family.

关 键 词：Leber＇s hereditary optic neuropathy mitochondrial DNA MUTATION HAPLOTYPE vision loss MODIFIER Chinese 

分 类 号：Q244[生物学—细胞生物学] Q78