外周血端粒酶活性测定对循环肺癌细胞的诊断价值  

Significance of telomerase activity in the diagnosis of circulating lung cancer cells

作　　者：邸志强 侯继申[2] 薛承岩[2] 杜新生[2] 杨亚平 

机构地区：[1]承钢集团职工医院内科,河北省承德市067002 [2]承德医学院附属医院,067000

出　　处：《疑难病杂志》2008年第11期669-670,共2页Chinese Journal of Difficult and Complicated Cases

基　　金：2007年河北省科学技术支撑计划项目(No.07276101D-2);2008年承德市科学研究与发展计划项目(No.200821127)

摘　　要：目的了解外周血液端粒酶活性检测诊断循环肺癌细胞的应用价值。方法外周血液标本应用端粒重复序列扩增—微孔板杂交—酶联免疫分析检测端粒酶活性。结果肺癌组血液端粒酶活性的阳性率显著高于非肿瘤组和健康组(P<0.01),Ⅲ、Ⅳ期的阳性率显著高于Ⅰ期(P<0.05或<0.01)。结论检测血液端粒酶活性有助于发现循环肺癌细胞。Objective To understand the clinical value of telomerase activity detection in peripheral blood in the diagnosis of circulating lung cancer cells. Methods Telomerase activity was detected by telomeroc repeat amplification protocol-hybridism-enzyme-linked immunosorbent assay in peripheral blood. Results The positive rate of telomerase activity in lung cancer group was much higher than the non-tumor group and the health group （ P 〈 0.01） ; the positive rate of the marker in stage Ⅲ and Ⅳ were much higher than in stage Ⅰ（ P 〈 0.05 or P 〈 0.01） in the lung cancer group. Conclusion Detection of telomerase activity in peripheral blood is important in the discovery of circulating lung cancer cells.

关 键 词：外周血液 端粒酶活性 循环肺癌细胞 诊断 

分 类 号：R734.2[医药卫生—肿瘤] R730.4[医药卫生—临床医学]