用回归法消除溶血对总蛋白测定的影响  

Eliminating the influence of hemolysis on total serum protein with regression method

作　　者：肖士海[1] 陈霞[1] 

机构地区：[1]盐城出入境检验检疫局,盐城224002

出　　处：《口岸卫生控制》2008年第5期29-30,共2页Port Health Control

摘　　要：目的溶血对总蛋白(TP)测定结果有正干扰作用,但干扰的具体情况及干扰有无规律性目前还没有多少人研究,该文旨在探讨出溶血对总蛋白测定的影响规律。方法用正常人混合血清将血红蛋白(Hb)标准液配制成系列标准溶血标本和一个非溶血标本(即混合血清标本),用双缩脲法测定这些溶血标本和非溶血标本的血清总蛋白,得出不同浓度溶血标本的TP增高值,然后用回归法对数据进行处理。结果发现不同程度的溶血对总蛋白测定结果的影响呈明显的正比例关系,相关系数r=0.9998。结论在日常工作中,碰到溶血标本,就可以用回归法很方便地消除溶血对总蛋白测定的影响,无需重新抽血测定。Objective Hemolysis has positive affect on total serum protein determination,whereas no one do research on the affect extent and its rule nowadays, finding out this rule is the aim of this paper. Methods Mixed normal human serum were prepared into a series of standard hemolysis specimens with hemoglobin standard solution, determine total serum protein with biuret, then process the test data with regression method. Results There is a positive correlation between different extent of hemolysis and total serum protein value, the correlation coefficient r = 0.9998. Conclusion The affect of hemolysis on total serum protein can be eliminated with regression method.

关 键 词：双缩脲 总蛋白(TP) 血红蛋白(Hb) 

分 类 号：R-33[医药卫生]