缬沙坦对兔自体移植静脉内膜增生及基质金属蛋白酶-2、组织基质金属蛋白酶抑制剂-2表达的影响  

Effect of valsartan on intima hyperplasia and expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in rabbit autologous vein graft

作　　者：仲崇俊[1] 蒋庚西[1] 

机构地区：[1]南通大学第二附属医院心胸外科,南通市226001

出　　处：《中华老年多器官疾病杂志》2008年第5期401-403,419,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：南通市社会发展计划资助项目(NoS2005023)

摘　　要：目的观察缬沙坦对兔颈部自体移植静脉内膜增生、基质金属蛋白酶-2(MMP-2)、组织基质金属蛋白酶抑制剂-2(TIMP-2)表达的影响,探讨AT_1受体拮抗剂干预移植静脉再狭窄的作用和机制。方法雄性新西兰大白兔20只行颈部自体静脉移植手术,对照组予普通饲料饲养,缬沙坦组予普通饲料+缬沙坦10mg/(kg·d)饲养。4周后取出静脉移植物行血管形态学检查;图像分析系统计算血管腔内膜的厚度、面积及中膜的厚度、面积;免疫组织化学方法检测MMP-2、TIMP-2表达情况。结果对比对照组,缬沙坦组移植静脉新生内膜厚度及面积明显降低,MMP-2表达降低,TIMP-2表达增加。结论缬沙坦可以抑制兔自体移植静脉新生内膜的形成,其机制可能与抑制内膜MMP-2表达、促进TIMP-2表达有关。Objective To investigate the effect of valsartan on intimal hyperplasia, the expression of matrix metalloproteinase-2 （MMP-2） and tissue inhibitor of metalloproteinase-2 （TIMP-2） in neointima, and to explain its mechanism of preventing stenosis in rabbit autotogous vein grafts. Methods Twenty male New Zealand rabbits were randomly divided into control group and treatment group with 10 in each. The rabbits underwent the operation of grafting external jugular veins into carotid arteries. The control group was fed with common feed, whereas the treat- ment group was fed with an admixture of valsartan 10mg/（kg· d） and common feed. Four weeks after the operation, the grafted veins were taken out for pathomorphologic examination. Thickness and area of the intima and media of the vein were calculated with image analyzer system, and expression of MMP-2, TIMP-2 in veins were examined with im- munohistochemistry reagent kits. Results Compared with the control group, the neointimal thickness and area were reduced in the treatment group. In the treatment group, the expression of MMP-2 was significantly lower while the expression of TIMP-2 was significantly higher than those in the control group. Conclusion Valsartan has remarkable inhibiting effects on intimal byperplasia in rabbit autologous vein grafts. The mechanism may be related with the decrease in level of MMP-2 and the increase in level of TIMP-2 in vein grafts.

关 键 词：血管紧张素Ⅱ1型受体拮抗剂 自体移植 静脉 基质金属蛋白酶2 组织基质金属蛋白酶抑制剂2 

分 类 号：R735.2[医药卫生—肿瘤] R654.306[医药卫生—临床医学]