肿瘤组织重构：脑肿瘤干细胞的作用  

Role of brain tumor stem cells in tumor tissue remodeling

作　　者：吴银艳[1,2] 黄强[1,2] 刁艺[1,2] 张全斌[1,2] 董军[1,2] 王爱东[1,2] 兰青[1,2] 

机构地区：[1]苏州大学附属第二医院神经外科,苏州215004 [2]苏州大学衰老与神经疾病实验室,苏州215123

出　　处：《中华神经医学杂志》2008年第11期1124-1126,共3页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30400457、30672164）

摘　　要：目的探讨脑肿瘤干细胞（BTSCs）移植瘤组织巾的肿瘤细胞、血管内皮细胞的起源问题，旨在阐明BTSCs在脑肿瘤组织重构中所起的作用。方法BTSCs球体接种到NC系裸小鼠右尾状核，在出现恶病质时处死，取移植瘤组织常规石蜡包埋、切片后进行HE染色和人类白细胞抗原（HLA）染色，在光学显微镜下观察。结果HE染色见肿瘤细胞广泛播散在宿主的脑组织中，HLA染色显示存在肿瘤细胞直接构成的血管。结论在BTSCs的裸小鼠移植瘤巾，肿瘤细胞存宿主脑内广泛分布并构成了血管；BTSCs可能转分化成肿瘤血管细胞，在脑胶质瘤组织重构中起重要作用。Objective To investigate the origin of tumor cells and vascular endothelial cells in intracranial brain tumor stem cell （BTSC） xenografts and elucidate the role of BTSCs in brain tumor tissue remodeling. Methods BTSC spheres were injected into the right caudate nucleus of nude mice, and the mice were sacrificed after the occurrence ofcaehexia to obtain the tumor tissue. Routine paraffin embedding, slicing, HE staining and human leucocyte antigen （HLA） staining of the tumor tissues were performed for observation of the tumor tissues under optical microscope. Results HE staining displayed dissemination growth of the tumor cells in the host brain tissue. HLA staining revealed the presence of blood vessels constituted directly by the tumor cells. Conclusion In the intracranial xenografts of BTSCs, the tumor cells are extensively distributed and constitute the blood vessels. The BTSCs play an important role in glioma tissue remodeling by differentiating into vascular endothelial cells in the tumors.

关 键 词：脑肿瘤 干细胞 移植瘤 组织重构 

分 类 号：R739.4[医药卫生—肿瘤]