大鼠冠脉原位血栓模型中纤维介素的表达及意义  

Expression and significance of fgl2 in a model of rat coronary microthrombosis in situ

作　　者：熊信林[1] 谢建[1] 王朝晖[1] 苏冠华[1] 汪艳[1] 刘辉[1] 王珏[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心研所,湖北武汉430022

出　　处：《中国病理生理杂志》2008年第11期2087-2091,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2007CB512000;No.2007CB512005)

摘　　要：目的:探讨在大鼠冠脉原位血栓模型中纤维介素(fgl2)的表达及意义。方法:雄性SPF级SD大鼠78只,随机分到模型组36只,假手术组36只和正常对照组6只,模型组与假手术组术后1h、1d、1周、2周、3周、4周各6只。模型组经主动脉根部注入月桂酸钠,假手术组注入生理盐水,正常对照组不予处理,分别用HE与纤维素快速染色(MMSB)、ELISA、RT-PCR法、免疫组化法观察冠脉微血管原位血栓的形成及血管性假血友病因子(vWF)与fgl2的表达。结果:模型组大鼠心肌微血管可见血小板纤维蛋白性原位血栓,有原位血栓形成的微血管数量显著增加;血浆中vWF水平明显升高;RT-PCR示fgl2表达明显增加,免疫组化示fgl2分布于心肌微血管壁。假手术组和正常对照组未见明显变化(P>0.05)。结论:在冠脉微血管内皮细胞功能紊乱的基础上,冠脉微血管壁fgl2表达显著增加并促进以血小板纤维蛋白沉积为主的原位血栓形成。AIM ： To explore the expression and significance of fibrinogen - like protein 2 prothrombinase/fibroleukin （fgl2） in a model of rat coronary microthrombosis. METHODS ： Seventy - eight Sprague - Dawley male rats were randomly assigned to model group （ n = 36）, sham operation group （ n = 36） for different time （ 1 h, 1 d, 1 week, 2 weeks, 3 weeks, 4 weeks） （n = 6, respectively） , and normal control group （n = 6）. After closing aorta, sodium laurate was injected into aortic root of the animals in model group. The animals in sham operation group were injected with normal saline for control. Microthromb were detected with HE staining and modified martius- sarlet- blue stains （MMSB）. yon Willebrand factor （vWF） was measured with ELISA. fgl2 mRNA and protein expressions were detected by RT - PCR and immunohistochemistry staining, respectively. RESULTS： Microthromb were observed in rat coronary microvasculature, vWF was increased in plasma, fgl2 was induced in model group compared to sham operation group （ P 〈 0. 05 ） , no significant changes were observed in sham operation group and normal control group （ P 〉 0. 05 ）. CONCLUSION： The functional disorder of endothelial cells induces the expression of fgl2 in the coronary microvasculature, fgl2 prompts the thrombosis with platelet and fibrin.

关 键 词：冠状动脉 原位血栓 纤维蛋白原样蛋白2凝血酶原酶 月桂酸类 

分 类 号：R363[医药卫生—病理学]