EETs下调在高脂诱导肥胖相关高血压中的作用  

Role of EETs downregulation in obesity-related hypertension induced by a high fat diet

作　　者：黄辉[1] 陈洁[1] 王景峰[2] 黄湖辉[1] 徐安平[1] 吕军[1] 王华敏[1] 

机构地区：[1]中山大学附属第二医院肾内科,广东广州510120 [2]中山大学附属第二医院心内科,广东广州510120

出　　处：《中国病理生理杂志》2008年第11期2113-2116,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30700304)

摘　　要：目的:探讨肾脏环氧二十碳烯酸(epoxyeicosatrienoic acids,EETs)在高脂饮食诱导的幼年型肥胖相关高血压发病机制中的作用。方法:以高脂饮食喂养3周龄SD雄性大鼠至12周龄,监测正常饮食与高脂饮食两组大鼠的体重与血压的变化,并以Western blotting和反相HPLC方法比较两组大鼠的的肾脏各部分EETs活性的差异。结果:高脂饮食大鼠体重在第8周龄,收缩压在第11周龄,均明显高于正常饮食组[(328±23)gvs(273±21)g,(153.0±8.6)mmHgvs(134.0±7.7)mmHg,P<0.05],而两组肾微小血管产生EETs的活性无差异;高脂饮食组肾皮质和乳头部产生EETs的活性低于正常饮食组[(75.4±9.2)nmol·g-1·min-1 vs(138.1±10.3)nmol·g-1·min-1,(55.8±6.2)nmol·g-1·min-1vs(121.6±11.3)nmol·g-1·min-1,P<0.05],同时Western blotting表明产生EETs的细胞色素P450(CYP)表达也出现相应的降低。结论:高脂饮食诱导的幼年型肥胖相关高血压的发生可能与肾皮质和乳头部的EETs下调有关。AIM ： To study the effect of renal epoxyeicosatrienoic acids （EETs） on juvenile rats with obesity related hypertension induced by high fat diet. METHODS： Sprague - Dawley male rats were fed with high fat diet from 3 - week old. The changes of weight and sBP between the rats of high fat diet and normal diet were compared. EETs activity was analyzed with RP HPLC and Western blotting in different parts of kidney. RESULTS ： Weight and sBP increased in high fat diet group at the eighth and eleventh weeks [ （328 ± 23） g vs （273.0±21.0） g, （ 153.0 ± 8. 6） mmHg vs （ 134. 0± 7.7 ） mmHg, P 〈 0. 05 ]. No significant change of the EETs activity of renal microvessels between two groups was observed. The EETs activity in cortex and papilla decreased in high fat diet group compared with that in normal diet group [ （75.4±9.2）nmol·g^-1·min^-1 vs （138.1±10.3）nmol·g^-1·min^-1, （55.8±6.2）nmol·g^-1·min^-1 vs （121.6±11.3） nmol·g^-1·min^-1, P 〈 0.05 ] , and this was confirmed by Western blotting. CONCLUSION ： These results demonstrate that juvenile rats with obesity related hypertension induced by high fat diet might be related to the downregulation of EETs activity in cortex and papilla.

关 键 词：高血压 肥胖症 花生四烯酸 细胞色素P450 

分 类 号：R363.1[医药卫生—病理学]