杂交瘤细胞克隆化培养及其分泌的单克隆抗体的纯化  

Clone Culture of Hybridoma Cells and Purification of Its Secreting McAb

作　　者：汪永和[1] 魏祥志 何明坤[1] 

机构地区：[1]合肥市第三人民医院,合肥230022

出　　处：《安徽卫生职业技术学院学报》2008年第5期81-82,共2页Journal of Anhui Health Vocational & Technical College

摘　　要：目的:纯化单克隆抗体。方法:先将分泌单抗N35的杂交瘤细胞P35作克隆化培养,再应用protein A-sepharose CL-4B制备免疫亲和层析柱,纯化单克隆抗体。结果:单抗纯化后电泳呈一条带,且保存良好的免疫活性。结论:有限稀释法对细胞进行克隆化培养是十分有效的,应用protein A-sepharose CL-4B亲和层析法可以有效地纯化单克隆抗体。Objective：To purify monoclonal antibody N-35.Methods： Firstly, to sub-clone hybridoma cell P-35 secreting monoclonal antibody N-35; then make affinity chromatography column with protein A-Sepharose CL-4B to purify monoclonal antibody N-35. Results： Purified monoclonal antibody N-35 is electrophoresised and can show single band and keep nicer immunocompetence. Conclusion： Limiting dilution used in sub-cloning cells is very effective. Affinity chromatography through protein A-Sepharose CL-4B can purify monoclonal antibody effectively.

关 键 词：有限稀释 纯化 亲和层析 单克隆抗体 N35 

分 类 号：R733[医药卫生—肿瘤]