节律基因mPer1对化疗药物阿霉素敏感性的影响  

Effects of Circadian Gene mPer1 on Therapeutic Sensitivity of Adriamycin

作　　者：段志青[1,2] 朱小云[1] 张宇[1] 汪宇辉[1] 刘延友[1] 郭慧玲[1] 肖静[1] 王正荣[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物医学工程实验室,成都610041 [2]中国医学科学院医学生物学研究所质量管理处

出　　处：《四川大学学报（医学版）》2008年第6期925-928,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30470623、30470684)资助

摘　　要：目的评价小鼠乳腺癌EMT6细胞中节律基因mPer1过表达对化疗药物阿霉素(ADM)敏感性的影响。方法将pcDNA3.1(+)-mPer1质粒转染至EMT6细胞中(EMT6-mPer1组),以pcDNA3.1(+)质粒为对照(EMT6-vect组),通过RT-PCR、Western Blotting检测转染效率;给予ADM处理,MTT法检测细胞生长情况,流式细胞术检测细胞凋亡及细胞周期的变化;RT-PCR检测凋亡相关基因mRNA的水平。结果在ADM处理后,与对照组比较,转染mPer1基因细胞表现为:S期比例增加,凋亡率升高〔EMT6-vect:(65.65±0.07)%;EMT6-mPer1:(72.35±0.57)%〕,细胞生长抑制率增加〔EMT6-vect:(42.18±5.73)%;EMT6-mPer1:(53.28±7.32%)〕及p53 mRNA表达量增加(EMT6-vect:0.48±0.08;EMT6-mPer1:1.18±0.02)。结论节律基因mPer1过表达可以提高该细胞对阿霉素的敏感性。Objective To study the effects of roper1 gene on the response of mammary carcinoma EMT6 cells to Adriamycin in vitro. Methods The eukaryotie expression vector pcDNA3, 1（＋）-mPer1 was transfeeted into the EMT6 cells （EMT6-mPer1）. The vector peDNA3. 1 （＋） transfeet was also performed to serve as the control （EMT6-veet）. The transfect efficiency was detected by RT-PCR and Western Blotting. The transfect cells were treated with Adriamyein in vitro. The apoptosis and distribution of cells in the cell cycle were analysed by FCM. The cell proliferation was detected by MTT assay. RT-PCR was used to show the mRNA expression of apoptosis-related genes. Results The rnPer1-transfected EMT6 cells revealed S phase arrest, increased rate of apoptosis [EMT6-vect： （65. 65±0. 07）%; EMT6-mPer1; （72. 35± 0. 57）%], decreased cell proliferation [EMT6-vect： （42.18±5.73）%; EMT6-mPer1： （53. 28±7.32%）%] and stronger expression of p53 mRNA in RT-PCR （EMT6-vect： 0.48±0.08; EMT6-mPer1： 1.18±0.02）. Conclusion mPer1 gene can improve the drug sensitivity of this cell line to ADM in vitro.

关 键 词：节律基因 mPer1 基因 阿霉素 药物敏感性 

分 类 号：R73-36[医药卫生—肿瘤]