Cycling and Tai Chi Chuan exercises exert greater immunomodulatory effect on surface antigen expression of human hepatitis B virus  

作　　者：CHEN Yu-yawn CHIANG Jasson CHEN Yu-jen CHEN Kung-tung YANG Rong-sen LIN Jaung-geng 

机构地区：[1]Department and Graduate School of Physical Education, National Taiwan Sport University, Taichung, Taiwan, China [2]Graduate Institute of Sport Coaching Science, Chinese Culture University, Taipei, Taiwan, China [3]Department of Medical Research, Department of Radiation Oncology, Mackay Memorial Hospital, Taipei, Taiwan, China [4]College of Humanities, Social and Natural Sciences, Ming Hsin University of Sciences and Technology, Taiwan, China [5]Department of Orthopaedics, College of Medicine, National Taiwan University & Hospital, Taipei, Taiwan, China [6]Graduate Institute of Chinese Medical Science, China Medical University. Taichung 404. Taiwan. China

出　　处：《Chinese Medical Journal》2008年第21期2172-2179,共8页中华医学杂志（英文版）

摘　　要：Background Both athletes with intensive exercise and aged people may have weakened immunity against virus infection. This study aimed to evaluate whether people undergoing aerobic exercises including competitive cyclists with moderate training （CMT） and middle-aged people practicing Tai Chi Chuan （TCC） exercise have higher immunity against hepatitis B virus than age-matched sedentary controls including college students （CSC） and middle-aged people （MSC）. Methods Human peripheral blood mononuclear cells from competitive cyclists and sedentary controls were stimulated by phytohemagglutinin （PHA） to prepare conditioned medium （MNC-CM） for the assessment of inhibitory effects on hepatitis B surface antigen （HBsAg） expression in human hepatoma Hep3B cells. Results The inhibitory effects on the relative HBsAg expression of CMT＇s and TCC＇s MNC-CM were greater than those of the controls. The CMT＇s MNC-CM prepared from 5 μg/ml PHA decreased HBsAg expression to 61.5%, whereas that of CSC remained at 83.8%. Similarly, this expression by treatment of TCC group＇ MNC-CM was 68.4% whereas that of MSC group was 84.3%. The levels of cytokines such as interferon-γ（IFN-γ）, tumor necrosis factor-α （TNF-α）, IFN-α and interleukin-1β （IL-1β） in the MNC-CM from the CMT and TCC groups were greater than those in the controls. Antibody neutralization of CMT＇s MNC-CM and addition of recombinant cytokines into CSC＇s MNC-CM indicated that IFN-γ, TNF-α and IFN-α had synergistic effects against HBsAg expression. Similar blocking effect was noted in TCC versus MSC groups. Conclusion These results suggest that the immunomodulatory response to suppress HBsAg expression in CMT and TCC with moderate aerobic exercise is greater than that in age-matched sedentary controls.Background Both athletes with intensive exercise and aged people may have weakened immunity against virus infection. This study aimed to evaluate whether people undergoing aerobic exercises including competitive cyclists with moderate training （CMT） and middle-aged people practicing Tai Chi Chuan （TCC） exercise have higher immunity against hepatitis B virus than age-matched sedentary controls including college students （CSC） and middle-aged people （MSC）. Methods Human peripheral blood mononuclear cells from competitive cyclists and sedentary controls were stimulated by phytohemagglutinin （PHA） to prepare conditioned medium （MNC-CM） for the assessment of inhibitory effects on hepatitis B surface antigen （HBsAg） expression in human hepatoma Hep3B cells. Results The inhibitory effects on the relative HBsAg expression of CMT＇s and TCC＇s MNC-CM were greater than those of the controls. The CMT＇s MNC-CM prepared from 5 μg/ml PHA decreased HBsAg expression to 61.5%, whereas that of CSC remained at 83.8%. Similarly, this expression by treatment of TCC group＇ MNC-CM was 68.4% whereas that of MSC group was 84.3%. The levels of cytokines such as interferon-γ（IFN-γ）, tumor necrosis factor-α （TNF-α）, IFN-α and interleukin-1β （IL-1β） in the MNC-CM from the CMT and TCC groups were greater than those in the controls. Antibody neutralization of CMT＇s MNC-CM and addition of recombinant cytokines into CSC＇s MNC-CM indicated that IFN-γ, TNF-α and IFN-α had synergistic effects against HBsAg expression. Similar blocking effect was noted in TCC versus MSC groups. Conclusion These results suggest that the immunomodulatory response to suppress HBsAg expression in CMT and TCC with moderate aerobic exercise is greater than that in age-matched sedentary controls.

关 键 词：competitive cyclists IMMUNOMODULATORY hepatitis B surface antigens 

分 类 号：R512.62[医药卫生—内科学]