地塞米松抑制哮喘小鼠SH2-Bβ蛋白表达  

The inhibitory effect of dexamethasone on the expression of SH2-Bβ protein in a murine model of asthma

作　　者：齐金萍[1] 王巧玲[1] 金韵[1] 胡颖[1] 

机构地区：[1]沈阳医学院解剖学教研室,辽宁沈阳110034

出　　处：《中国药理学通报》2008年第11期1501-1504,共4页Chinese Pharmacological Bulletin

基　　金：辽宁省教育厅基金资助项目(No20060890);沈阳医学院优秀人才基金资助项目(No20073022)

摘　　要：目的探讨地塞米松对哮喘小鼠肺及内脏感觉传入系统(C7-T5脊神经节及对应的脊髓后角)SH2-Bβ表达的抑制作用。方法用卵蛋白法制备BABL/c小鼠哮喘模型,应用免疫组织化学和免疫印迹法,观察SH2-Bβ在哮喘小鼠肺及内脏感觉传入部位的表达及地塞米松对其表达的影响。应用小鼠肺功能仪检测气道阻力的变化。结果在肺组织、C7-T5段脊神经节及相应节段的脊髓后角内,哮喘组SH2-Bβ表达明显高于对照组和地塞米松组(P<0.01)。哮喘组气道阻力明显高于正常组及地塞米松组(P<0.01)。结论地塞米松抑制哮喘小鼠SH2-Bβ的表达可能是激素治疗哮喘的机制之一。Aim To explore the inhibitory effect of dexamethasone on the expression of SH2-Bβ in the lung and the visceral sensory afferent system （C7-T5 spinal ganglia and the corresponding posterior horn of the spinal cord） of asthmatic mice. Methods Murine model of asthma of BABL/c mice was induced by ovalbumin in vivo. By means of immunohistochemistry and Western blot, the expression of SH2-BI3 in the lung, CT-T5 spinal ganglia and corresponding spinal cord was detected and the inhibitory effect of dexamethasone on it was observed. Inspiratory airway resistance was measured with AniRes 2003 lung functionsystem. Results The expression of SH2-Bβ in the lung, C7-T5 spinal ganglia and the corresponding spinal cord of the asthmatic mice was much higher than that of the control group and the dexamethasone group （P 〈 0. 01 ）. The inspiratory airway resistance was much higher in asthmatic group than that of the control group and the dexamethasone group （P 〈0.01 ）. Conclusion The inhibition of the expression of SH2-Bβ by dexamethasone might be one of the mechanisms in the treatment of asthma.

关 键 词：SH2-BΒ 地塞米松 肺 脊神经节 脊髓 哮喘 小鼠 

分 类 号：R-332[医药卫生] R322.35