蜡样芽孢杆菌B-02菌株培养基及培养条件的优化  

Optimizing Culture Medium and Culture Conditions of Bacillus cereus B-02

作　　者：刘婧[1] 马汇泉[1] 董瑾[1] 杨晓[1] 

机构地区：[1]山东理工大学生命科学学院,山东淄博255049

出　　处：《山东农业科学》2008年第7期54-57,共4页Shandong Agricultural Sciences

基　　金：山东省教育厅研究基金(T04c08)

摘　　要：为研究培养条件对蜡样芽孢杆菌(Bacillus cereus)B-02菌株生长和对番茄灰霉病菌(Botrytis ci-nerea)抑菌活性的影响,分别采用分光光度计法、琼脂孔扩散法和杯碟法进行Bacillus cereusB-02菌株的菌体量和抑菌活性的测定。结果表明,B-02菌株的最佳培养条件为:装液量50 ml(250 ml三角瓶),接种量1%(V/V),转速140 r/min,温度32℃,初始pH值为9.0的NB培养基中培养36 h;在此条件下B-02菌株抑菌活性比初始条件提高16.22%。In order to study the influence of culture conditions on the growth and antimicrobial activity of B. cereus strain B -02 against Botrytis cinerea, the methods of spectrometer, agar diffusion bioassay and well test were used respectively to quantify the bacterium as well as its antimicrobial activity. The best culture conditions were 50 ml NB medium in 250 ml flask with 1% inoculum concentration（ V/V）, 140 r/min rotation speed, 32℃, pH 9.0 and 36 hours. The antimicrobial activity of B - 02 had been increased 16.22% over the beginning.

关 键 词：番茄灰霉病 蜡样芽孢杆菌B-02 抑菌活性 培养条件 

分 类 号：S436.412.13[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]