金复康对大鼠大肠癌癌前病变的改善作用及尿液代谢物研究  

Effect of Jinfu Kang to experimental precancerous colon lesions and urinary metabolites in rat

作　　者：邱云平[1] 苏明明[1] 吴大正[2] 赵爱华[1] 刘玉敏[1] 贾伟[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]上海中医药大学,上海201203

出　　处：《中国中药杂志》2008年第22期2653-2657,共5页China Journal of Chinese Materia Medica

基　　金：国家科技部国际合作项目(2006DFA02700);上海市重点学科建设项目(T0301)

摘　　要：目的:考察二甲肼诱发大肠癌癌前病变大鼠的尿液中内源性小分子代谢物谱的变化过程,以及中药金复康干预下大鼠代谢谱的变化过程。方法:利用基于氯甲酸乙酯衍生、GC-MS技术的代谢组学方法。结果:经主成分分析表明,模型组大鼠的代谢谱具有随时间逐步偏离起始点的趋势,而金复康干预组大鼠的代谢谱则表现出先偏离再回归起始点的代谢谱变化轨迹,正常组大鼠的代谢谱在实验过程中没有出现明显的变化。对影响模型组大鼠与正常组大鼠代谢谱分离的权重变量进行鉴定和研究,发现三羧酸循环、色氨酸循环、多胺代谢等代谢通道的波动,以及肠道菌群代谢的紊乱与二甲肼诱导的大肠癌癌前病变相关。结论:代谢组学研究表明,金复康能有效逆转二甲肼诱导的大肠癌癌前病变大鼠的代谢谱的偏移,代谢组学的研究结果与病理实验结果相一致。Objective： To profile urinary metabolite variations from 1, 2-dimethylhydrazine （DMH）-induced precancerous coion rats, Jinfu Kang treated rats and healthy controls. Method： We used ethyl chloroformate derivatization and gas chromatographymass spectrometry （GC-MS） based metabonomic method to analyze rat urines, Result： The time-dependent variations of metabolite profile showed a progressive deviation of the metabolism in the model group from the initial pattern over time and a systemic recovery of the metabolism in the treatment group, which is consistent with the histological results. The in-depth analysis indicated that the disorder of tricarboxylic acid cycle （TCA）, tryptophan metabolism, polyamine metabolism and gut flora structure were associated with DMH intervention. Conclusion： Metabolic study revealed that Jinfu Kang can effectively reverse metabolic departures in DMH-induced precancerous colon rat, which is consistent with pathological results.

关 键 词：二甲肼 大肠癌癌前病变 代谢组学 模式识别 金复康 

分 类 号：R285.5[医药卫生—中药学]