瑞氏木霉QM9414利用蔗渣发酵产纤维素酶的研究  

Study on production of cellulase using bagasse by Trichoderma reesei QM9414

作　　者：伍红[1,2] 秦天莺[1] 谭德勇[2] 农向[3] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]云南大学生命科学学院,云南昆明650091 [3]乐山师范学院化学与生命科学学院,四川乐山614000

出　　处：《云南大学学报（自然科学版）》2008年第6期620-624,640,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：西南民族大学自然科学基金项目资助(23444)

摘　　要：以蔗渣为培养基料,通过单因子及正交试验,对瑞氏木霉QM9414固体发酵产纤维素酶的产酶条件进行了探讨.其优化的产酶条件为:甘蔗渣2.5 g,麸皮1 g,加含7.5 g/L(NH4)2SO4的Mandels营养液14 mL(干物质(g)与水(mL)的比例为1∶4),调初始pH 4.0,30℃发酵120 h.在此优化条件下,每克干曲产纤维素酶酶活力可达8.26 U.Utilizing bagasse as substrate the culture conditions for therma reesei QM9414 was studied through single factor experiments and orthogonal test. The results showed that the optimal culture conditions were as follows： medium prepared from lg bagasse, 2.5 g wheat bran and 14mL Mandels nutritional liquid containing 7.5 g/L（ NH4）2SO4,adjustment of initial pH to 4.0, at 30 ℃ for 120h. Under these optimal fermentation conditions, cellulase activity reached 8.26 U/g dry medium.

关 键 词：蔗渣 瑞氏木霉QM9414 正交实验 固态发酵 培养条件优化 

分 类 号：Q936[生物学—微生物学]