大鼠大脑皮层细胞条件培养液促进骨髓间充质干细胞向神经样细胞分化的研究  

Study on Effect of Conditioned Medium from Cerebral Cortex Cultures on Promote Neurogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

作　　者：周俊松[1] 董运海[1] 钱燕翔[1] 徐路尧[1] 宋琳[1] 宋雪慧[1] 辛莲[1] 张焕相[1] 

机构地区：[1]苏州大学医学部细胞生物学教研室,江苏省干细胞研究重点实验室,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2008年第5期689-691,695,F0002,共5页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30671041);973项目(2005CB623906);江苏省高校自然科学重大基础研究项目(06KJA18025);江苏省高校自然科学基金一般项目(05KJB310115)

摘　　要：目的建立大脑皮层细胞条件培养液促进骨髓间充质干细胞向神经样细胞分化的方法。方法原代培养并鉴定骨髓间充质干细胞;用条件培养基、无血清培养基、DMEM分别诱导骨髓间充质干细胞6h和24h;用免疫荧光染色的方法鉴定由骨髓间充质干细胞分化而来的神经样细胞。结果骨髓间充质干细胞表达CD106、CD90、CD29和CD44标记物,不表达CD71、CD45和CD34,回收得到的条件培养基可以诱导骨髓间充质干细胞分化为神经样细胞,这些神经样细胞的免疫荧光染色呈β微管蛋白Ⅲ、胶质原纤维酸性蛋白、巢蛋白阳性,且阳性比例明显高于实验对照组,差异有高度统计学意义(P<0.001)。结论大脑皮层细胞条件培养液能促进骨髓间充质干细胞向神经样细胞的分化。Objective To establish the method of that conditioned medium from cerebral cortex cultures promote neurogenic differentiation of bone marrow mesenchymal stem cells（BMSCs）. Methods Primary culture and identification of BMSCs： BMSCs were induced by conditioned medium, serum-free medium and DMEM for 6 h or 24 h; The differentiated neuron-like cells derived from BMSCs were characterized by specific immunofluorescence staining. Results BMSCs at passage five were positive for CD106,CD90,CD29 and CD44, negative for CD71,CD45 and CD34. The conditioned medium retrieved from the mixed cultures of newborn rat cerebral cortex could induce the BMSCs to differentiate into neuron-like cells. These neuron-like cells expressed β-Ⅲ-tubulin,GFAP and nestin, characteristics of neurons,astrocytes and neural progenitors respectively and the percentage of the positive cells was obviously higher than that of the controls （P〈0.001）. Conclusion Conditioned medium from cerebral cortex cultures can promote neurogenic differentiation of BMSCs.

关 键 词：骨髓间充质干细胞 神经样细胞 条件培养基 细胞分化 

分 类 号：R730.264[医药卫生—肿瘤]