Microtox技术在印染废水污染监测中的应用研究  

Application of Microtox Technique in Monitoring of Dye House Waste Pollution

作　　者：王宾香[1] 王慧[1] 郑天凌[1,2] 

机构地区：[1]厦门大学生命科学学院、应用与环境微生物研究所 [2]近海海洋环境科学国家重点实验室(厦门大学),福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第6期869-873,共5页Journal of Xiamen University：Natural Science

基　　金：福建省重大专项前期研究项目(2006YZ1023);厦门市科技计划项目(3502Z20073009)资助

摘　　要：利用发光细菌毒性检测技术(Microtox)研究了Microtox技术对印染废水毒性检测的条件,活性染料及降解中间产物对发光细菌的毒性反应,并对柠檬酸杆菌降解活性染料降解过程进行了监测.结果表明:Microtox技术可以有效地监测活性染料及其微生物降解中间产物的相对毒性;活性染料的毒性远大于代谢途径中间产物的毒性;活性染料降解过程中培养液萃取物的毒性逐渐降低,但上清液的毒性出现先下降而后又增大的趋势,推测是上清液中存在的中间产物和其它组成成分及pH变化综合作用的结果.The proper condiction of dye house waste toxicity measuring was studied by Microtox test in this paper, the Microtox test was also used to assay the toxicity of reactive dye and its metabolites,and the process of Citrobaeter to degrade reactive dye from dye house waste was detected in this paper. It has been found that the biotoxicity of reactive dye and its metabolites were successfully assayed by Microtox test. Reactive dye was more toxic than its metabolites, such as sulfanilic acid, orthanilic acid, metanilic acid and aniline. Microtox test demonstrated a decrease in the toxicity of culture extracts from the culture containing reactive dye, and a decrease followed with an increase in the toxicity of culture supernatants over the incubation period. The metabolites and change of the component of the medium and pH value must be responsible for the toxicity of the culture supernate together.

关 键 词：发光菌 Microtox技术 生物毒性 环境检测 

分 类 号：X172[环境科学与工程—环境科学]