养殖常用抗菌药物对凡纳滨对虾外壳膜N-乙酰-β-D-氨基葡萄糖苷酶活力的影响  

Antibacterial Medicines on the Activity of N-acetyl-β-D-glucosaminidase from Prawn(Litopenaeus vannamei)

作　　者：杜娟[1] 安刚[1] 谢晓兰[2] 黄乾生[1] 王烨[1] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]泉州师范学院化学与生命科学学院,福建泉州362011

出　　处：《厦门大学学报（自然科学版）》2008年第6期883-886,共4页Journal of Xiamen University：Natural Science

基　　金：福建省科技重点项目(2006N0067);厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室开放课题(2007108)资助

摘　　要：研究几种抗菌药物对凡纳滨对虾壳膜N-乙酰-β-D-氨基葡萄糖苷酶(NAGase,EC 3.2.1.52)活力的影响.结果表明,诺氟沙星和磺胺甲噁唑对该酶活力有不同程度的抑制作用,盐酸环丙沙星对此酶有激活作用,而青霉素钾、链霉素、庆大霉素、卡那霉素、磺胺嘧啶等对酶活力基本上没有影响;磺胺甲噁唑的抑制作用最为显著,导致酶活力下降50%的抑制剂浓度(IC50)为0.094 mg/mL.抑制作用动力学研究结果表明,磺胺甲噁唑对酶的抑制作用为混合型可逆抑制,其抑制常数KI为5.98 mg/mL,对酶-底物络合物的抑制常数KIS为13.08 mg/mL.该研究对凡纳滨对虾的人工养殖具有一定的参考价值.The effects of some antibacterial medicines on the hydrolysis of pNP-NAG catalyzed by N-acetyl-β-D-glucosaminidase （NAGase,EC 3. 2.1.52） from prawn （Litopenaeus vannamei） were studied. The results indicated that norfloxacin and acetylsulfamethoxazole could inhibit the enzyme activity in different extent, while penicilin,streptomycin,gentamicin,kanamycin and sulfadiazine had hardly any effects on the enzyme activity. The inhibitory effect of acetylsulfamethoxazole was the most potent. The value of IC50, the inhibitor concentrations leading to 50 % enzyme activity lost, was estimated to be 0. 094 mg/mL. The inhibitory kinetics of acetylsulfamethoxazole on the enzyme was further studied. The results showed that acetylsulfamethoxazole was reversible inhibitor of mixed-type, and the inhibition constants K1 and K1S were determined to be 5.98 mg/mL and 13.08 mg/mL, respectively.

关 键 词：凡纳滨对虾 N-乙酰-Β-D-氨基葡萄糖苷酶 抗菌类药物 酶活力 抑制机理 

分 类 号：Q356.1[生物学—遗传学]