大鼠非酒精性脂肪性肝纤维化形成过程中骨桥蛋白的表达及其意义  

The expression and significance of osteopontin in the development of nonalcoholic fatty liver fibrosis in rats

作　　者：刘萍[1] 陈东风[1] 王军[1] 艾正琳[1] 樊丽琳[1] 王晓敏[1] 严丽丽[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所消化内科,重庆400042

出　　处：《中华肝脏病杂志》2008年第11期831-834,共4页Chinese Journal of Hepatology

摘　　要：目的通过观察非酒精性脂肪性肝纤维化形成过程中肝组织内骨桥蛋白（OPN）的变化规律，探讨OPN在非酒精性脂肪性肝纤维化形成过程中的作用。方法选用纯系Wistar雄性大鼠56只，体质量180-200g，随机分为正常对照组和高脂饮食4、8、12、16、20、24周组（其中每组各8只）。肝组织常规进行HE及Masson三色胶原染色，免疫组织化学染色检测肝组织a-平滑肌肌动蛋白的表达，RT-PCR、Western blot观察肝组织中OPN动态变化。结果与正常对照组相比，高脂饲料组大鼠肝脏内OPN含量明显升高，随着脂肪肝程度的加重，OPN表达逐渐增强，mRNA及蛋白质表达比较，F值分别为7．30和7．15，P值均〈0．01；与a-平滑肌肌动蛋白表达及肝组织胶原纤维面积百分比呈显著正相关（r值分别为0．94和0．82，P值均〈0．01）。结论在非酒精性脂肪性肝纤维化形成过程中，OPN在肝组织中表达显著上调，可能在非酒精性脂肪性肝纤维化的形成过程中发挥重要作用。Objective To investigate the changes of osteopontin （OPN） in the liver tissues during nonalcoholic fatty liver fibrosis in rats and to explore the effect of OPN in the development of nonalcoholic fatty liver fibrosis. Methods Fifty-six male Wtstar rats were randomly divided into a control group （8 rats） and a high-fat diet group. The high-fat diet group was divided into 6 subgroups （8 rats in each subgroup） with high-fat feedings for 4, 8, 12, 16, 20 or 24 weeks. Conventional histochemical, HE, Masson-trichrome and immunohistochemical staining for a -smooth muscle actin （ a-SMA） were performed with the liver histological preparations. The expression of OPN was detected with reverse transcription and polymerase chain reactions and Western blot. Results Levels of OPN in liver tissues in rat nonalcoholic fatty liver fibrosis induced by high-fat diet were significantly increased over those in the control group （F = 7.15, P 〈 0.01）. OPN expressions were closely correlated with a -SMA and nonalcoholic fatty liver fibrosis, and correlation coefficients of the two groups were 0.94 and 0.82, and both P values were less than 0.01. Conclusion Expression of OPN increases dramatically in the livers during the development of nonalcoholic fatty liver fibrosis, and OPN may play an important role in this event.

关 键 词：脂肪肝 非酒精性 肝纤维化 骨桥蛋白 

分 类 号：R575.2[医药卫生—消化系统] R575.5[医药卫生—内科学]