抑制炎症反应治疗急性肺损伤的实验研究  

Treatment of Acute Lung Injury by Anti-inflammatory Response

作　　者：颜春松[1] 赵年贵[2] 杨夏玲[1] 罗俊明[1] 王石生[1] 饶纬华[1] 

机构地区：[1]南昌大学第二附属医院呼吸科,南昌330006 [2]厦门市第二医院呼吸科,福建厦门361021

出　　处：《江西医学院学报》2008年第5期20-23,28,共5页Acta Academiae Medicinae Jiangxi

摘　　要：目的通过观察急性肺损伤(ALI)大鼠血液和肺组织中IL-6、IL-10、TNF-α、NF-κΒ等炎性细胞因子的变化,探讨抑制炎症反应在治疗急性肺损伤中的作用。方法应用气管内滴入内毒素(LPS)复制大鼠急性肺损伤模型。将48只大鼠随机分成4组:对照组(N组)、LPS组、N-乙酰半胱氨酸(NAC)治疗组(NAC组)、NAC联合地塞米松(Dex)治疗组(NAC+Dex组),采用免疫印迹法(Western Blotting)检测NF-κΒ的含量,采用RT-PCR检测肺组织中IL-6、IL-10、TNF-α、NF-κΒmRNA的表达,并采用放免检测血清及支气管肺泡灌洗液(BALF)中的IL-6、TNF-α的含量。结果LPS组大鼠肺病理可见明显的肺损伤表现,NAC组及NAC+Dex组肺组织病理改变明显减轻;NAC组及NAC+Dex组肺NF-κB,肺IL-6、IL-10、TNF-α、NF-κΒmRNA表达水平及血清和BALF中的IL-6、TNF-α含量明显高于N组(P<0.01),而明显低于LPS组(P<0.05)。结论NAC、Dex等可以抑制炎症反应,减少炎性介质的释放从而减轻肺损伤的程度,达到防治急性肺损伤的目的。Objective To investigate the treatment effect of anti-inflammatory response on the expressions of IL-6 ,IL-10,TNF-α,NF-κB mRNA in the lung and the levels of IL-6,TNF-α in serum and BAI,F in rats with lipopotysaccharide-induced acute lung injury（ALI）. Methods A total of 48 Spradue Dawley rats were divided into 4 groups randomly： normal saline control group（N group）, lipopolysaccharid group （LPS group）, N-acetylcysteine group （NAC group）, NAC associate with dexamethasone group （NAC＋Dex group）. The levels of TNF-α,IL-6 were determined by radioimmunoassay. The expresions of IL-6,IL-10,TNF-α,NF-κB mRNA were detected by RT- PCR,NF-κB were determined Western-Blotting. Results Compared with those in the nomml saline control group,the expression levels of IL-6,IL-10,TNF-α,NF-κB mRNA and NF-κB in the injured lungs and the levels of IL-6,TNF-α in serum and BALF increased signifieantly（P〈0.01） ; Compared with those in the LPS group,NAC group and NAC＋Dex group decreased significantly （P〈0.05）. Conclusion N-acetylcysteine and dexamethasone can inhibit the production of these inflammatory cytokines in vivo and the mRNA expression in the lung tissues. Anti-inflammatory response may prevente acute lung injury.

关 键 词：急性肺损伤 炎症反应 细胞因子 动物 实验 大鼠 

分 类 号：R-332[医药卫生]