平阳霉素加地塞米松治疗血管瘤的实验研究  

An Experimental Study of Pingyangmycin and Dexamethasone Co-treatment on Hemangioma

作　　者：郭竹秀[1] 刘征宇[2] 郑威[3] 吴红宣[1] 

机构地区：[1]南昌大学第一附属医院皮肤科,南昌330006 [2]南昌大学医学院外科总论教研室,南昌330006 [3]赣南医学院附属医院皮肤科,江西赣州341000

出　　处：《江西医学院学报》2008年第5期38-40,44,F0003,共5页Acta Academiae Medicinae Jiangxi

摘　　要：目的观察平阳霉素和地塞米松对兔耳缘静脉的组织学改变,探讨平阳霉素加地塞米松治疗血管瘤的机制。方法将20只新西兰兔随机分为4组,每组5只,即生理盐水组、平阳霉素组、地塞米松组、平阳霉素加地塞米松组。各组动物注药后观察其全身及局部反应,并分别于注药后7、14、21、283、5 d光镜下观察其组织学变化,7、14 d分取部分标本固定后于电镜下观察超微结构。结果(1)肉眼观:注药1h后各组动物均无发热,呼吸及心率平稳。(2)光镜下:生理盐水组注药后各时段均无明显变化;地塞米松组注药后14 d血管内皮细胞肿胀,无血栓形成;平阳霉素组注药7 d后血管腔扩张,血管内皮细胞增生肥大,14 d部分血栓形成,21、28、35 d管壁增厚,管腔未完全闭塞;联合组7 d血管腔血栓形成,14 d血管壁水肿及增厚,21、28、35 d血管壁结构消失,管腔闭塞。(3)电镜下:生理盐水组注药后各时段均无明显变化;地塞米松组注药14 d后血管内皮细胞肿胀;平阳霉素组注药7 d线粒体扩张,胞质不均,14 d内皮细胞空泡变性,线粒体肿胀,管腔内见血浆蛋白纤维;联合组注药7d血管内皮细胞线粒体肿胀,粗面内质网扩张,吞噬小体增多,14 d血管壁胶原层裸露,血管腔内见血浆蛋白纤维。结论平阳霉素和地塞米松可使血管腔闭塞,且周围组织反应轻,可作为血管瘤尤其海绵状血管瘤硬化治疗的一种较理想的选择。Objective To investigate the histological changes of pingyangmycin and dexamethasone co-treatment on rabbit＇s ear marginal vein. To further discuss the underlying mechanism of pingyangmycin and dexamethasone in the treatment of hemangioma. Methods 20 New Zealand rabbits were randomly divided into 4 groups （n = 5）, including control group, pingyangmycin treatment group, dexamethasone treatment group, pingyangmycin and dexamethasone co-treatrnent group. Local reaction and general reaction would be observed and histological changes were observed under light microscope on day 7,14,21,28 and 35 after drug treatment in every group. Some tissue samples were taken out and fixed for electron microscope assay on day 7 and 14. Resuits （1）By using naked eye：the animals in every group hadn＇t been occurred fever,the breath and heartbeat was normal after drug treatment one hour. （2）By light microscopy：After injection, the control group had no significant changes in every period. In the dexamethasone treatment group,endothelial swelling appeared at 14 d,but the blood flow was not blocked. In pingyangmycin treatment group, lumen dilated, endothelial cells were proliferative and hypertrophy at 7 d; Part of the thrombosis was observed in the lumen of blood vessel at 14 d;At 21,28,35 d,the wallof the blood vessel had more layers,but the lumen of the vessel was still remain. In co-treatment group,thrombus was observed at 7 d;in 14 d,the vessel wall was edema and thickening ; in 21,28, 35 d,the endomembrane structure was disappeared and blood vesse lumen was blocked. （3）By Electron microscopy： After injection, the control group had no significant changes in every period. In the dexamethasone treatment group,endoplasmic reticulum swelling was visible in vascular endothelial cells at 14 d. In pingyangmycin treatment group,mitochondria swelling happened at 7 d, and cytoplasm was disparity; the endothelial cells was vacuolar degeneration, mitochondria was swelling,and plasma protein remain saw inside the lumen of

关 键 词：平阳霉素 地塞米松 实验模型 血管瘤 动物 实验 兔 

分 类 号：R-332[医药卫生]