动物源性ω-3 PUFAs对耐多柔比星人卵巢癌细胞系A2780逆转耐药性研究  

Study the Drug Resistance Reversion of Animal Origin ω-3 Polyunsaturated Fatty Acids to Human Ovarian Carcinoma Cell Line A2780 Holding Doxorubicin(DXR) Resistance

作　　者：黄雯雯[1] 

机构地区：[1]汕头大学医学院第一附属医院药剂科,广东汕头515041

出　　处：《河北医学》2008年第11期1273-1276,共4页Hebei Medicine

摘　　要：目的:通过人卵巢癌细胞及多柔比星耐药株的体外细胞毒性实验,探讨两种动物源性ω-3多不饱和脂肪酸对耐多柔比星人卵巢癌细胞系A2780逆转耐药性的作用。方法:采用浓度梯度倍增法诱导建立耐药细胞A2780/DXR,并与亲代细胞A2780进行耐药性比较。将耐药细胞A2780/DXR和亲代细胞A2780分别分为空白对照组、VRP逆转对照组(VRP10μmol/ml)、DHA10μmol/ml组、DHA20μmol/ml组、EPA10μmol/ml组、EPA20μmol/ml组,用动物源性ω-3多不饱和脂肪酸对A2780/DXR进行逆转耐药。结果:亲代细胞A2780组,DHA20μmol/ml和EPA20μmol/ml的逆转效果与VRP10μmol/ml的逆转效果具有显著性差异(P<0.05);耐药细胞A2780/DXR组,DHA20μmol/ml的逆转效果与VRP10μmol/ml的逆转效果具有显著性差异(P<0.05)。结论:动物源性ω-3多不饱和脂肪酸DHA、EPA均能有效逆转耐药株对DXR的敏感性,且有明显的剂量依赖性。其中尤以EPA(20μmol/ml)的逆转作用更加明显。Objective： To approach the drug resistance reversion effect of two kinds of animal origin ω -3 polyunsaturated fatty acids, to human ovarian carcinoma cell line A2780 holding doxorubicin （DXR） resistance, by the vitro cytotoxicity experiment of human ovarian cancer cell and doxorubicin persister. Methods ：To induce and set the drug resistance cell A2780/DXR by concentration gradient multiplication method, and do drug resistance comparison with parent cell A2780. The drug resistance cell A2780/DXR and parent cell A2780 were divided respectively into blank control group, VRP reverse control group （ VRP 10μmol/ ml）, DHA 10μmol/ml group, DHA 20μmol/ml group, EPA 10μmol/rrd group, EPA 20μmol/ml group. To reverse the drug resistance of A2780/DXR by animal origin ω-3 polyunsaturated fatty acids. Results： In parent cell A2780 group, the reverse effect of DHA 20μmol/ml and EPA 20μmol/ml had significant difference （P 〈 0.05） , compared with VRP 10μmol/ml. In the drug resistance cell A2780/DXR group, the reverse effect of DHA 20μmol/ml had significant difference （P 〈 0.05） , compared with VRP 10μmol/ml. Conclusion： Animal origin ω -3 polyunsaturated fatty acids DHA and EPA both reverse effectively the sensitivity of persister to DXR. Both have conspicuous dose dependent. Especially, the reverse effect of EPA 20μmol/ml is more conspicuous.

关 键 词：ω-3多不饱和脂肪酸 多柔比星 人卵巢癌细胞 逆转耐药性 

分 类 号：R737.31[医药卫生—肿瘤]