survivin反义核酸抑制胶质瘤细胞增殖的实验研究  

Experimental study of the inhibition effect of survivin antisense RNA on proliferation of human glioma cell line SHG-44

作　　者：王志成[1,2] 王海霞[1,2] 郭德玉[1] 刘丽梅[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038 [2]解放军第371中心医院病理科,河南新乡453000

出　　处：《重庆医学》2008年第22期2570-2571,2574,共3页Chongqing medicine

摘　　要：目的探讨survivin反义核酸对胶质瘤细胞增殖的影响。方法用脂质体介导法将survivin反义核酸真核表达载体pIRES2-EGFP-SVVas转染入人胶质瘤SHG-44细胞系中,G418筛选阳性克隆后,用Immunoblot检测survivin蛋白表达,光、电镜观察细胞形态学改变,MTT法检测细胞生长曲线,流式细胞仪检测细胞周期分布。结果pIRES2-EGFP-SVVas质粒转染SHG-44胶质瘤细胞后细胞survivin蛋白表达水平降低,而细胞由卵圆形变为长梭形,细胞突起明显增多、增长,细胞增殖活性明显降低,细胞生长受到抑制。结论survivin反义核酸能通过抑制SHG-44细胞survivin蛋白表达,抑制该细胞增殖。Objective To study the effect of survivin antisense RNA on proliferation of SHG-44 ceils. Methods Survivin antisense RNA was successfully transfeeted into SHG-44 cells by lipofectamine. The positive cell clone was screened by G418, and pro rein expression of survivin was detected by immunoblot assay. The morphological changes were observed with light and electron microscope. The cell growth curve was analyzed by MTT method. The cell cycle was analyzed by flow eytometry. Results The protein expression level of survivin was decreased and cell growth and proliferation was inhibited in the SHG-44 cells transfected by plRES2-EGFP-SVVas plasmid than that of control group. The cell shape transformed into fusiform shape from orbicular-ovate,and the cell pustutes became numerous and long. Conclusion Survivin antisense RNA can inhibit the proliferation of glioma SHG-44 cells by suppressing the expression of survivin protein.

关 键 词：SURVIVIN 反义核酸 胶质瘤 细胞增殖 

分 类 号：R739.41[医药卫生—肿瘤]