鸭瘟病毒强毒株在感染鸭实质器官内的增殖与分布  

Replication kinetics of duck plague virus virulent strain in parenchymatous organs of experimentally infected ducklings

作　　者：杨晓燕[1,2] 程安春[1] 汪铭书[1] 齐雪峰[1,3] 郭宇飞[1] 葛忠源[1] 黄永成[1] 张素辉[1] 胡骑[1] 于波[1] 周登春[1] 陈孝跃[1] 

机构地区：[1]四川农业大学动物科技学院禽病防治研究中心动物疾病与人类健康四川省重点实验室,四川雅安625014 [2]大理学院生命科学与化学学院,云南大理671000 [3]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国兽医学报》2008年第11期1254-1258,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30471297);国家科技攻关重大资助项目(2004BA901A03);教育部“新世纪优秀人才支持计划”资助项目(NCET-04-0906);四川省重点建设学科资助项目(SZD0418)

摘　　要：鸭瘟病毒(DPV)CHv强毒株经皮下注射、滴鼻和口服3种途径分别感染20日龄天府肉鸭,于攻毒后10、30、60、90min以及4、12、48、72h和9、15d每组分别剖杀2只鸭,采集心、肝、脾、肺、肾、脑、胸腺、法氏囊、哈德氏腺等实质器官,应用TaqMan-MGB探针实时荧光定量PCR对DPV在这些器官的分布和增殖进行检测。结果表明,DPV分布到具体器官的速度与感染的途径、鸭的解剖结构密切相关,其中皮下注射是DPV分布到各实质器官速度最快的途径。30min于皮下感染鸭的肝、脾、胸腺、法氏囊、哈德氏腺、肺、脑、肾,口服感染鸭的肺和法氏囊,滴鼻感染鸭的心脏和哈德氏腺均检测到DPV-DNA;90min所有受检样品中检测到DPV-DNA。鸭抗DPV感染的免疫器官的重要性依次是脾、胸腺、法氏囊和哈德氏腺,30min内DPV-DNA分布到脾、胸腺、法氏囊的速度和数量决定了DPV感染的潜伏期和疾病的严重程度。不同途经感染鸭的相同器官在同一时间内的DPV-DNA拷贝数大多以皮下感染鸭为最高。DPV致死鸭的法氏囊和肾是DPV-DNA含量最高的实质器官。The kinetics of virus load was examined in ducklings infected with virulent duck plague virus （DPV） by the mucosal or systemic route at 20 days of age. Two infected birds were chosen randomly for sampling at each of ten sampling time between 10 min and 15 days postinoculation （p. i. ） ,and the distribution and replication of DPV in the parenchymatous organs, including heart, liver, spleen, lung, kidney, brain, thymus, bursa of Fabricius and glands of Harder,were examined using real-time quantitative TaqMan-MGB polymerase chain reaction. The results showed that the distribution of DPV in muhiple organs examined have a close correlation with the infected administration and the anatomized structure of duck,and the levels of viral DNA could be first detected in organs of subcutaneously infected birds. The significant numbers of virus genomes were detected in subcutaneously infected organs at 30 min p. i. ,including liver, spleen, thymus, bursa, Harder gland, lung, brain and kidney, and were also detected in lung and bursa of orally infected birds,and heart and Harder gland of nasally infected birds. By 90 rain p. i. , the significant levels of DPV were detected in all organs examined independent of the infected administration. The successive importance of immune organs in resistance the DPV infection was spleen, thymus, bursa, and Harder gland. The spread and viral levels of DPV in organs, including spleen, thymus, bursa, have a close correlation with the progression of disease. The virus loads in individual organs of subcutaneously infected birds were relatively higher at the same time point, compare with that in orally or nasally infected birds. The peak levels of DPV in bursa and kidney of dead ducklings were higher among organs examined.

关 键 词：TaqMan—MGB探针 实时荧光定量PCR 鸭瘟病毒强毒 人工感染 鸭 实质器官 

分 类 号：S852.65[农业科学—基础兽医学]