水牛体细胞连续核移植的效果  

Effects of buffalo somatic cell serial nuclear transfer

作　　者：杨素芳[1] 陈自洪[1,2] 谢英[1,3] 成俊萍[1,4] 陆凤花[1] 石德顺[1] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530005 [2]广西壮族自治区人口和计划生育研究中心,广西南宁530021 [3]河北医科大学,河北石家庄050017 [4]广西壮族自治区人民医院,广西南宁530021

出　　处：《中国兽医学报》2008年第11期1360-1362,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30460090);广西青年基金资助项目(桂科青0542022)

摘　　要：为探讨水牛体细胞连续核移植的效果,以水牛胎儿成纤维细胞为核供体,进行了水牛体细胞连续核移植。结果显示,连续核移植的融合率显著高于原代核移植(87.9%vs76.2%,P<0.05),但两者之间的分裂率和囊胚率没有显著差异(P>0.05);这说明水牛体细胞核移植胚胎可被再次克隆而不降低其发育能力,水牛体细胞连续核移植是可行的。To examine the effects of somatic cell serial nuclear transfer （SCSNT） on buffalo somatic cell nuclear transfer （SCNT）,SCSNT was conducted with the buffalo fetal cells as the donor cells. The results showed, the fusion rate of SCSNT was higher than that of original somatic cell nuclear transfer （87.9% vs 76.2% ,P〈0.05） ,but the cleavage rate and blastocyst development rate were not different from each other. It indicated that,buffalo SCNT embryos can be recloned without reduce of their development ability and that the buffalo SCSNT should be feasible.

关 键 词：水牛 体细胞核移植 连续核移植 

分 类 号：S814.8[农业科学—畜牧学]