东北梅花鹿染色体限制性内切酶显带  

Restriction Endonucleases Bandings of Metaphase Chromosome of Northeastern Sika Deer (Cervus nippon hortulorum)

作　　者：蒋德梅[1] 蔡志华[1] 韩莉[1] 陶红梅[1] 姜计 温兴福 樊汝权 

机构地区：[1]重庆师范大学生命科学学院,重庆400047 [2]重庆市永川松既职业高级中学

出　　处：《四川动物》2008年第6期1107-1110,共4页Sichuan Journal of Zoology

基　　金：重庆师范大学校级项目(XLY012);重庆市科委自然科学基金(CSTC,2006BB1260)

摘　　要：采用限制性内切酶HaeⅢ、HindⅢ分别处理梅花鹿中期染色体标本,发现HaeⅢ处理标本21h左右,显出类似G带、C带带型,第1、4、11、18、27、28、31对染色体的结构异染色质区对HaeⅢ敏感而浅染,而HindⅢ处理标本18h左右能诱导梅花鹿中期染色体显出类似G带、C带,第2、6、15、18、29、32对染色体的结构异染色质区对HindⅢ较敏感而浅染,性染色体Y对HaeⅢ敏感呈浅染,性染色体X对HindⅢ敏感呈浅染。表明东北梅花鹿12对常染色体和两条性染色体结构异染色质在这两种酶处理时表现出异质性。The metaphase chromosomes of northeastern sika deer were treated with Hae m and Hind Ⅲ in this paper. The metaphase chromosomes of northeastern sika deer displayed G-like bands and C bands after treated with HaeⅢ for 21 hours, and the constitutive heterochromatin of No. 1, No. 4, No. 11, No. 18, No. 27, No. 28, No. 31 were sensitive to Hae Ⅲ and were dyed lightly. The metaphase chromosomes of northeastern sika deer displayed G-like bands and C bands after treated with Hind Ⅲ for 18 hours, and the constitutive heterochromatin of No. 2, No. 6, No. 15, No. 18, No. 29, No. 32 were sensitive to Hindm and were dyed lightly. Sex chromosome Y was sensitive to HaeⅢ and was dyed lightly, and sex chromosome X was sensitive to Hind Ⅲand was dyed lightly. The results indicated the heterogeneity of constitutive heterochromatin of twelve pairs of autosomes and two sex chromosomes of northeastern sika deer when treated with Haem and HindⅢ.

关 键 词：东北梅花鹿 限制性内切酶 染色体 显带 

分 类 号：Q55[生物学—生物化学]