茶树自交花粉管在活体花柱中的不亲和性观察  

Observation on the Self-incompatibility of Pollen Tubes in Self-pollination of Tea Plant in Style in vivo

作　　者：王郁[1] 江昌俊[1] 张和禹[1] 

机构地区：[1]安徽农业大学教育部农业部茶叶生物化学与生物技术重点实验室,安徽合肥230036

出　　处：《茶叶科学》2008年第6期429-435,共7页Journal of Tea Science

基　　金：安徽省教育厅自然科学研究计划重点项目(2004kjl37zd)

摘　　要：采用以苯胺蓝染色的荧光显微技术对茶树无性系品种龙井43活体花柱上(中)的自交和异交花粉(管)生长特性进行观察。结果发现,自交花粉可以在柱头上正常萌发生长;授粉后24h内自交与异交花粉管生长特性无明显差异,24h之后自交花粉管生长速度明显慢于异交,最后在花柱基部停止伸长,且伴随产生花粉管顶端形态异常,荧光异常明亮等不亲和(受抑制)现象。由此进一步证实茶树自交花粉管生长受抑制的形态学表现与植物配子体自交不亲和性类型特征相符合。Germination of pollens and growth of pollen tubes were observed in vivo after self- or cross-pollination in the style of clonal tea cultivar Longjing43 using fluorescence microscopy by aniline blue staining. The results showed that pollens in self-pollination germinated normally, and it was the same as that in cross-pollination. There was no significant difference in growth characteristics of pollen tubes between self- and cross-pollination during 24 hours after pollinating,but thereafter the growth speed of pollen tubes in self-pollination was slower than that in cross-pollination and was finally stoped in the style base, and meanwhile there were some incompatibility-phenomenons that the form of pollen tube apex was abnormal and fluoresced more obviously. It was suggested that the inhibited morphological manifestations of pollen tubes in self-pollination accorded with the characteristics of floral gametophytic self-incompatibility.

关 键 词：茶树 自交不亲和性 花粉管 荧光 自交 

分 类 号：S571.1[农业科学—茶叶生产加工] Q944.56[农业科学—作物学]