沙利度胺对人肾癌细胞增生及碱性成纤维细胞生长因子表达的影响  

Effects of thalidomide on proliferation of human renal cancer cell and expression of basic fibroblast growth factor

作　　者：姜文华[1] 刘鹏飞[2] 李小东[1] 

机构地区：[1]天津医科大学第二医院放疗科,300211 [2]天津医科大学附属肿瘤医院内一科

出　　处：《肿瘤研究与临床》2008年第11期724-726,共3页Cancer Research and Clinic

摘　　要：目的观察沙利度胺对人肾癌细胞株786—0增生及碱性成纤维细胞生长因子（bFGF）表达的影响。方法将细胞分成沙利度胺6．25、25、100ug／ml组及对照组；应用MTT方法测定细胞抑制率；半定量RT-PCR和流式细胞术分别从mRNA和蛋白水平观察沙利度胺对786—0细胞bFGF表达的作用。结果沙利度胺在6．25～100ug／ml范围内能明显抑制786—0细胞增生，作用48、72h，IC50分别为46．42、19．56ug／ml。凋亡率从12．43％增加到30．30％，并伴随bFGF表达水平下降，以25ug/ml对bFGF抑制最显著。结论沙利度胺能抑制肾癌细胞bFGF的表达，促进凋亡，抑制细胞增生。Objective To elucidate the effects of thalidomide on proliferation of human renal cell line 786-0 and the expression of basic fibroblast growth factor （bFGF） in this cell line. Methods Cell was treated with different doses of thalidomide（6.25 ug/ml, 25 ug/ml, 100 ug/ml） respectively or normal saline as control; cell survival rate was analyzed by MTT assay. The mRNA level of bFGF was detected by reverse transeriptase-polymerase chain reaction （RT-PCR）. bFGF protein expression in 786-0 cells was detected by flow cytometry （FCM）. Results Thalidomide, ranging from 6.25 ug/ml to 100 ug/ml, suppressed the proliferation of 786-0 cell line in vitro significantly. After application of thalidomide for 48 and 72 hours, the IC50 was 46.42 ug/ml and 19.56 ug/ml respectively. Apoptosis rate increased from 12.43 % to 30.30 %, accompanying with reducing expression of bFGF. Application of thalidomide （25 ug/ml） induced the most significant inhibition to the bFGF in the ceil line. Conclusion Thalidomide down-regulates bFGF expression, inhibit the proliferation , and induce apoptosis in 786-0 cell line.

关 键 词：细胞系 肿瘤 成纤维细胞生长因子 肾肿瘤 沙利度胺 

分 类 号：R737[医药卫生—肿瘤]