MMP-1、MMP-3、TIMP-1对外伤性PVR的影响研究  

Research on the effect of MMP-1,MMP-3,TIMP-1 on the traumatic PVR

作　　者：洪玉[1,2] 徐国兴[2] 

机构地区：[1]福建医科大学附属第二医院,中国福建省福州市350005 [2]福建医科大学附属第一医院,中国福建省福州市350005

出　　处：《国际眼科杂志》2008年第11期2195-2198,共4页International Eye Science

基　　金：中国卫生部科研基金资助项目(No.WKJ2005-2-013);福建省自然科学科研基金项目(No.C0510015);福建医科大学教授发展基金课题(No.2006-js6033)~~

摘　　要：目的:观察外伤性PVR大鼠和外伤后应用人工合成基质金属蛋白酶抑制剂(GM6001)中大鼠视网膜组织MMP-1、MMP-3及TIMP-1在不同病程中的表达变化。方法:将180只SD大鼠随机分为正常对照组、外伤性PVR组和外伤后应用GM6001组。应用免疫组化法在不同时间对各组大鼠视网膜组织MMP-1、MMP-3及TIMP-1的表达进行定性、定位、半定量检测。结果:1)MMP-1、MMP-3、TIMP-1主要表达于视网膜组织的视锥视杆层、视网膜外网状层、视网膜内网状层、神经纤维层。2)各个亚组在正常对照组、外伤后应用GM6001组MMP-1、MMP-3微弱表达。3)MMP-3在外伤性PVR组1,3,7d显著表达,MMP-1在外伤性PVR组3,7,14d显著表达(P<0.05)。TIMP-1在外伤性PVR组与外伤后应用GM6001组的各个亚组均有明显表达(P<0.01)。MMP-3/TIMP-1的比率在外伤性PVR组1,3,7d增高(P<0.05)。MMP-1/TIMP-1的比率在外伤性PVR组3,7,14d增高(P<0.05)。结论:MMP-1、MMP-3与PVR形成的病理过程有关,TIMP-1与二者特异性结合抑制其活性。GM6001通过调节MMP-1/TIMP-1和MMP-3/TIMP-1达到平衡,能抑制PVR的发生和发展,干预PVR的发展进程。AIM- To investigate the expression of MMP-1, MMP-3 and TIMP-1 during the course of traumatic PVR treated with GM6001 and without GM6001. METHODS： Totally 180 SD rats were divided randomly into three groups： normal control group, the traumatic PVR group, the traumatic PVR treated with GM6001 group. The expression of MMP-1, MMP-3 and TIMP-1 were qualitative and semiquantitative analysised with immunohistochemistry at different times. RESULTS. 1 ） Immunohistochemistry showed that the expression of MMP-1, MMP-3 and TIMP-1 were mainly located in the photoreceptor cells layer, out plexiform layer, inner plexiform layer and nerve fiber layer. 2） The expression of MMP-1 and MMP-3 in the normal group and the traumatic PVR treated with GM6001 group were weak at all time. 3） The expression of MMP-3 in the traumatic PVR group was strong at day 1, 3 and 7. The expression of MMP-3 in the traumatic PVR group was strong at day 3, 7 and 14 （P〈 0. 05）. The expression of TIMP-1 in the traumatic PVR group and the traumatic PVR treated with GM6001 group were strong at all time （P〈0.01）. The rate of MMP-3/TIMP-1 in the traumatic PVR group increased at day 1,3 and 7 （P〈0.05）. The rate of MMP-1/TIMP-1 in the traumatic PVR group increased at day 3, 7 and 14 （P〈0.05）. CONCLUSION： MMP-1 and MMP-3 play key role in the course of traumatic PVR. TIMP-1 binds specifitly to MMP-1 and MMP-3 and suppressed their activity. GM6001 plays an important role in the course of traumatic PVR prevention and treatment by regulating the rate of MMP-1/ TIMP-1 and MMP-3/TIMP-1.

关 键 词：基质金属蛋白酶-1 基质金属蛋白酶-3 基质金属蛋白酶抑制剂-1 GM6001 外伤性增生性玻璃体视网膜病变 免疫组化 

分 类 号：R779.1[医药卫生—眼科]