TiO_2纳米薄膜影响晶状体上皮细胞和巨噬细胞生长的体外研究  

TiO_2 nanometer thin film influence the growth of lens epithelium cells and macrophages in vitro

作　　者：郑帆[1] 翁景宁[1] 

机构地区：[1]福建医科大学附属协和医院眼科,中国福建省福州市350001

出　　处：《国际眼科杂志》2008年第11期2199-2202,共4页International Eye Science

基　　金：中国福建省自然科学基金资助项目(No.C0510017);中国福建省联合攻关项目(No.WKJ2005-2-003)~~

摘　　要：目的:观察经长波紫外线UVA(365nm)激发处理,TiO2纳米薄膜对体外培养的牛晶状体上皮细胞和小鼠腹腔巨噬细胞的抑制效应,研究该薄膜影响表面细胞生长的特性。方法:在玻片上涂覆TiO2纳米薄膜,并以未镀膜玻片为对照。将两种玻片在不同强度(0.102和0.825mW/cm2)的UVA下,照射0,10,20,30,40min,观察玻片表面的晶状体上皮细胞和巨噬细胞生长的数量和状态。实验用MTT比色法评估晶状体上皮细胞量,并以AO-EB染色法观察其形态;用瑞氏染色后计数法测定巨噬细胞量,并以扫描电镜观察其形态。结果:镀TiO2纳米薄膜玻片在0.102mW/cm2的UVA激发40min或0.825mW/cm2的UVA激发30min时,表面存活的细胞量比未镀膜玻片显著减少(P<0.01),且晶状体上皮细胞出现AO-EB染色的凋亡和坏死特征,巨噬细胞出现质膜破裂、细胞崩解的扫描电镜表现。激发时间越长,TiO2纳米薄膜上的细胞量越少,且0.825mW/cm2的光源较0.102mW/cm2的光源更能激发TiO2的杀伤作用。结论:TiO2纳米薄膜在UVA的激发照射后,能使其表面的晶状体上皮细胞和巨噬细胞发生凋亡或坏死。与照射时间呈时效关系。AIM. To observe the depressive effect of TiO2 nanometer thin film on lens epithelium cells （LEC） and macrophages （MФ） in vitro when it is activated during UVA illumination, and to investigate its influence on surface cellular proliferation. METHODS： TiO2 membrane was formed on glass substrate and studied with naked glass plate as control. TiO2 coated plate or naked plate were exposed to different magnitude of UVA （0. 102mW/cm^2 and 0. 825mW/cm^2） for sorts of period （0, 10, 20, 30, and 40 minutes）. During the exposure, number and viability of LECs and MФs on the surface of plates were observed. The assay of MTT Colorimetric and Wright＇s staining were respectively used to estimated the number of LECs and MФs, and Acridine Orange/Ethidium Bromide （AO-EB） staining as well as scanning electron microscope（SEM） were separately applied to observe the morphology of LECs and MФs. RESULTS.. The number of LEC and Me on TiO2 membrane was inferior to control group significantly （ P〈 0.01 ）, while it was exposed to UVA with light intensity of 0. 102mW/cm^2 for 40 minutes or 0. 825mW/cm^2 for 30 minutes. At the same time, LEC showed the characteristic of apoptosis and necrosis by AO-EB staining, and Me displayed the sign of cellular membrane rupture and disintegration in SEM. However, most of the cells on the surface of naked glass plate behaved normally under UVA raying. The longer TiO2 thin film got irradiated, the fewer living cells on it were. And the lighthouse containing larger energy induced more predominance provocation to TiO2 membrane. CONCLUSION： TiO2 nanometer thin films can produce the photocatalytic killing effect with UVA irradiation, and promote the apoptosis and necrosis of LEC and MФon its surface. The photocatalytic killing effect of TiO2 nanometer thin films is based on UVA raying, and related to luminous intensity and exposure time. TiO2 nanometer thin films may inhibit the growth of LEC and MФ on its surface with UVA excitation.

关 键 词：晶状体 上皮细胞 巨噬细胞 二氧化钛 表面修饰 生物相容性 光催化剂膜 

分 类 号：R776[医药卫生—眼科]