VEGF基因多态性与肺癌危险因素的关系  

Relationship between genetic polymorphism of VEGF and risk factor to lung cancer

作　　者：梁婧[1] 刘晓琳[1] 孙殿水[1] 刘海荣[1] 胡伟[1] 曲爱忠[1] 李岩[1] 

机构地区：[1]山东大学附属千佛山医院肿瘤诊疗中心,山东济南250014

出　　处：《中国癌症杂志》2008年第11期811-815,共5页China Oncology

基　　金：山东省科委自然科学基金项目资助(2005ZX04)

摘　　要：背景与目的:血管内皮生长因子(vascular endothelial growth factor,VEGF)是有力的血管生成介质,在肿瘤的生长及转移中起重要作用。本研究探讨VEGF基因多态性与肺癌危险因素的关系。方法:以病例对照研究方法,采用PCR-RFLP技术,对171例肺癌患者和172例健康对照者的VEGF基因-2578C/A及936C/T位点基因型进行检测,明确两个位点基因型。并采用PHASE110软件构建这两个多态性位点的个体单倍体型。以非条件Logistic回归校正混杂因素,并进行多态性与肺癌风险关联性的统计学分析。结果:携带至少1个-2578A等位基因的个体与携带-2578CC基因型的个体相比,肺癌发病风险降低(P=0.001,OR=0.303,95%CI 0.153～0.601)。性别分层分析显示:携带-2578CA+AA基因型男性患者其肺癌发病风险降低。936C/- 2578C与936C/-2578A两种单倍体分布差异有显著性(P=0.016,OR=0.317,95%CI 0.124～0.809;P=0.018,OR=0.547,95%CI 0.331～0.903)。病理分层显示:与其他对照组单倍体相比,C-C单倍体个体其腺癌发病风险降低(P=0.004,OR=0.237,95%CI 0.090～0.627)。结论:VEGF基因多态性是肺癌危险因素的风险因素。Background and purpose： Vascular endothelial growth factor（VEGF） is a potent anglogenlc mediator and angiogenesis has important effects on tumor growth and metastasis. The present study was to investigate the relationship between genetic polymorphism of VEGF and heredity risk factor of lung cancer. Methods： VEGF genotypes were determined by PCR-RFLP method in 171 patients with lung cancer and 172 healthy controls. Software PHASE 1.0 was used to construct the haplotypes of every individual. Unconditional logistic regression model was used to analyze the statistical association of genotypes or haplotypes in the two groups adjusted by gender and age. Results： Individuals with at least one -2578A allele had a significantly decreased risk of lung cancer compared with those carrying -2578CC genotype. When the analyses were stratified by gender, the combined -2578 CA and AA genotype, were associated with a considerably reduced risk of lung cancer（P=0.001,OR=0.303,95%CI=0.15 3-0.601）. The distribution of the two haplotypes（936C/-2578C and 936C/-2578A） among overall lung cancer cases was significantly different from that among the controls（P=0.016,OR=0.317,95%CI=0.124-0.809 and P=0.018,OR=0.547, 95%CI=0.331-0.903）. When the cases were categorized by tumor histology, the distribution of C-C haplotype in the adenocarcinoma（AC） group was associated with a substantially lowered risk of AC（P=0.004,OR=0.237, 95%CI=0.090- 0.627）, compared with the reference haplotypes. Conclusion： VEGF polymorphism may be a critical risk for the genetic risk factor to lung cancer.

关 键 词：肺肿瘤 VEGF基因 危险因素 基因多态性 

分 类 号：R730.231[医药卫生—肿瘤] R734.2[医药卫生—临床医学]