人参二醇组皂苷对人前列腺癌DU145细胞增殖与凋亡的影响  

Apoptosis and its mechanism of DU145 prostatic cancer cells induced by panoxadiol saponin

作　　者：刘艳波[1,2] 赵丽娟[1] 郭亚雄[1] 韩向北[1] 李德龙[1] 董妍[1,3] 赵雪俭[1] 

机构地区：[1]吉林大学前列腺疾病防治研究中心,吉林长春130021 [2]北华大学基础医学院病理生理学教研室 [3]美国杜兰大学Roswell Park癌化学防治教研室

出　　处：《中国男科学杂志》2008年第10期9-12,共4页Chinese Journal of Andrology

基　　金：吉林省人民政府人才开发基金资助课题;(2006JD02)

摘　　要：目的观察人参二醇组皂苷(panoxadiol saponin PDS)体外对DU145前列腺癌细胞增殖及凋亡的影响。方法采用MTT比色法检测不同浓度PDS对DU145细胞增殖活力的影响。吖啶橙染色观察PDS诱导细胞凋亡情况;流式细胞仪分析细胞周期及凋亡,并计算细胞平均凋亡率;细胞免疫化学和Western blot技术观察细胞内激活的caspase3的表达。结果经中(100mg/L)、高(200mg/L)剂量的PDS处理后,DU145细胞生长受到不同程度抑制,且呈现剂量依赖性;吖啶橙荧光染色可见经中、高剂量PDS处理后的细胞呈明显凋亡形态,并随药物剂量增加,凋亡细胞数增多;流式细胞术结果显示,PDS可明显提高细胞的凋亡率,呈剂量依赖关系;免疫细胞化学染色和Western blot结果,随着PDS剂量增加,细胞内激活的caspase3的表达上调。结论 PDS体外对DU145细胞增殖具有明显的抑制作用,其机制可能与诱导细胞凋亡有关。Objective To approach the effect of panoxadiol saponin （PDS） on DU145 prostatic cancer cells proliferation and apoptosis and study its mechanism in vitro. Methods After the DU145 cells were treated with different doses of PDS the cells viability was examined by MTT test. The techniques of acridine orange staining and flow cytometry were used to analyze apoptotic cycle and calculate the apoptotic rates. The active caspase3 expression was detected with immunocytochemistry and Western blot. Results After DU145 cells were treated with middle （100mg/L） and high（200mg/L） doses of PDS, the growth of cell line was inhibited with dose-dependent obviously. Acridine orange staining found that the cells were apoptotic state treated with middle and high dose of PDS. Flow cytometry results showed that the cell apoptotic rates were elevated with dose-dependence when treated by PDS. The expression of active caspase3 was upregulated by immunocytochemistry and western blot techniques. Conclusion PDS can inhibit proliferation of DU145 prostatic cancer cells and posesses significant anti-tumor effect in vitro. The mechanism may be that PDS activate the pathway of apoptosis.

关 键 词：细胞凋亡 人参二醇皂苷 前列腺肿瘤 细胞周期 

分 类 号：R737.25[医药卫生—肿瘤]