重症肌无力CD45RA、CD45RO胸腺细胞亚群及相关细胞因子表达分析  

Expression of CD45RA and CD45RO thymocyte subset and related cytokine in patients with myasthenia gravis

作　　者：李倩如[1] 杜英[1] 赵航[1] 高峰[2] 张清勇[3] 张钦宪[4] 

机构地区：[1]郑州大学基础医学院微生物学与免疫学教研室,河南郑州450001 [2]河南省医学科学研究所,河南郑州450052 [3]郑州大学第二附属医院胸外科,河南郑州450000 [4]郑州大学基础医学院组织胚胎学教研室,河南郑州450001

出　　处：《细胞与分子免疫学杂志》2008年第11期1066-1067,1071,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30570625);河南省自然科学基金资助(511042700)

摘　　要：目的:探讨胸腺细胞异常分化与重症肌无力发生的关系。方法:采用基因芯片对多种白细胞介素、干扰素及其受体mRNA表达进行分析,采用流式细胞术测定胸腺细胞CD45RA、CD45RO的表达率,采用免疫组化对胸腺组织切片CD45RA、CD45RO表达及分布进行检测。结果:MG患者IL-1R、IL-4R、IFNγR1、IFNγR2、IL-6、IL-8表达水平显著低于对照组;IL-10RB表达水平显著高于对照组;IL-1、IL-2、IL-4、IL-10、IL-7、IFN-α、IFN-β、IFN-γ表达水平在MG和对照组均很低,无显著差异;MG患者CD56+胸腺细胞百分率(0.56±0.33)显著低于对照组(1.78±0.69),MG患者CD45RO+、CD1a+细胞显著高于对照组。免疫组化和RT-PCR也有相同结果。结论:MG患者胸腺细胞发育过程中CD45RO+细胞向CD45RA+T细胞转变存在异常。AIM： To study the relationship between the abnormal differentiation thymocytes and the pathogenesis of myasthenia gravis （MG）. METHODS： The gene expression of some ILs, IFN and their receptors was examined by cDNA array. The percentage of CD45RO＋ and D45RA＋ thymocytes was measured by flow cytometry （FCM）. The expression and distribution of CD45RA and CD45RO in the samples of thymus tissues were observed by immunohistochemistry. RESULTS： The levels of IL-1R, IL-4R, IFNγR1, IFNγR2, IL-6 and IL-8 in the patients with MG were lower than those of normal controls and there were significant differences between them. But the levels of IL-1, IL-2, IL-4, IL-10, IL-7, IFN-α, IFN-βand IFN-γ showed no significant changes in patients with MG in comparison with normal controls. The percentage of CD56^＋ thymocytes in MG patients（0.56±0.33）was significantly lower than that in normal controls（1.78±0.69）.The CD45RO^＋ and CD1a^＋ in MG patients increased more significantly those in normal controls. The immunohistochemistry and RT-PCR showed the same result. CONCLUSON： Aberrant transformation from CD45R0^＋T to CD45RA^＋T has been found in the development of thymocytes of the patients with MG.

关 键 词：白细胞介素 CD45RA CD45RO 重症肌无力 胸腺 

分 类 号：R392.11[医药卫生—免疫学]