转移相关蛋白与乳腺癌转移潜能的相关性分析  

Investigation of the correlation between a group of metastasis-associated proteins and metastatic potency in breast carcinoma

作　　者：房爱菊[1] 高永生[1] 李盛梅[1] 刘文君[1] 孟斌[1] 

机构地区：[1]山东大学齐鲁医院病理科,山东济南250012

出　　处：《中国普通外科杂志》2008年第11期1079-1083,共5页China Journal of General Surgery

基　　金：山东省自然科学基金(Y2007C015)

摘　　要：目的探讨与肿瘤转移相关的蛋白在乳腺浸润性导管癌中的表达及其意义。方法应用组织芯片技术及SP免疫组化方法检测247例乳腺浸润性导管癌中α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的表达,并分析它们与乳腺癌临床病理特征的关系。结果(1)在浸润性导管癌中,α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的阳性表达率分别为70.0%,61.5%,57.5%,57.1%;与正常组织比较差异有统计学意义(P<0.001);(2)单因素分析显示α-B晶体蛋白,CD44v6,MMP-2的表达与淋巴结转移呈正相关(r=0.317,0.623,0.705),而TIMP-2的表达与之呈负相关(r=-0.532);(3)多因素分析显示淋巴结转移与α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的表达相关,该4项指标联合对淋巴结转移的预测率达89.5%。结论α-B晶体蛋白,CD44v6,MMP-2,TIMP-2的表达情况与乳腺浸润性导管癌的转移密切相关,通过对原发瘤多种转移相关蛋白的检测可以预测乳腺癌的转移能力。Objective To investigate the expression and the significance of a group of metastasis-associated proteins in invasive duetal breast carcinoma （IDC）. Methods Tissue microarray containing 247 IDC specimens was constructed. The expressions of α-B erystallin, CD44v6, MMP-2 and TIMP-2 were detected by immunohistochemistry, and the relation between the expression of these proteins and the clinicopathologic character was analyzed. Results （ 1 ） The expression rates of α-B crystallin, CD44v6, MMP-2 and TIMP-2 in IDC were 70. 0% , 61. 5% , 57. 5% and 57. 1% respectively, and significantly higher than those of normal breast tissues （ P 〈 0. 001 ） ; （ 2 ） Univariate analysis indicated that there were significant positive correlations between the expression of α-B crystallin, CD44v6, MMP-2 and lymph node metastasis of breast carcinomas （ r value is 0. 317 , 0. 623 and 0. 705 respectively ） , and significant negative correlations with TIMP-2 （ r = - 0. 532 ）. （ 3 ） Logistic stepwise regression analysis indicated that the expression of α-B erystallin, CD44v6, MMP-2 and TIMP-2 were significantly related with the lymph node metastasis, and the predicted percentage for the metastasis was 89. 5% by these four proteins together. Conclusions The expressions of α-B crystallin, CD44v6, MMP-2 and TIMP-2 was significantly correlated with the metastasis of IDC. It is possible to estimate the metastatic potency of breast carcinoma by testing metastasis-associated proteins.

关 键 词：乳腺肿瘤 肿瘤转移 组织芯片 因素分析 

分 类 号：R737.9[医药卫生—肿瘤]