基质金属蛋白酶2 mRNA在人喉鳞癌组织中的定位及临床意义  

Localization and Clinical Significance of Matrix Metalloproteinase-2 mRNA Protein in the Human Laryngeal Squamous Cell Carcinoma Tissue

作　　者：温蓓[1] 陈立[2] 梁传余[3] 李国义[3] 郑艳[3] 欧华[3] 

机构地区：[1]四川省人民医院耳鼻咽喉科,四川成都610072 [2]上海交通大学附属新华医院耳鼻咽喉头颈外科,上海200092 [3]四川大学华西医院耳鼻咽喉科,四川成都610041

出　　处：《华西医学》2008年第4期695-697,共3页West China Medical Journal

摘　　要：目的:探讨基质金属蛋白酶2(MMP-2)mRNA在人喉鳞状细胞癌中的定位及临床意义。方法:对50例喉癌患者的原发灶50例,癌旁组织34例,正常组织22例,采用原位杂交方法检测MMP-2 mRNA在喉癌组织中的定位及表达情况,通过图像分析研究其表达量与临床病理参数(T分期、病理分级、淋巴结转移)的关系。结果:MMP-2 mRNA在肿瘤细胞和基质细胞的胞浆中表达,其表达量在原发灶、癌旁(淋巴结)、正常组织中的表达呈递减趋势;与肿瘤的病理分级、T分期、淋巴结的转移无关。结论:MMP-2 mRNA在喉癌组织的肿瘤细胞和基质细胞的胞浆中表达,在原发灶中表达最高,其量的表达与病理分级、T分期、淋巴结的转移无关。可能是肿瘤浸润转移的一个早期信号。Objective：To study the localization and expression of matrix metalloproteinase-2（MMP-2）mRNA in human laryngeal squamous cell carcinoma tissue.Methods：The amount of expression of 50 laryngeal carcinoma patients,50 cases primary tissues lateral carcinoma,lymph node,20 cases normal tissue was analyzed by the immunohistochemical analysis.The MMP-2 protein in ISH（immunohistochemistry）was evaluated by the image analysis.In addition,the correlation between the MMP-2 mRNA expression and clinicopathological features（pathology grades,T stages,lymph node metastasis）wasevaluated.Results：The MMP-2mRNA was observed in carcinoma cell and stromal cytoplasm,Although the MMP-2mRNA expression in laryngeal carcinoma tissues was high than the lateral tissues,it was not correlated with clinicopathological features（the tumor T stage,pathology grades,lymph node metastasis）.Conclusion：The MMP-2mRNA was observed in carcinoma cell and stromal cytoplasm.MMP-2mRNA expression in the primary tissues in laryngeal carcinoma was the highest among these samples,it maybe an early information of carcinoma invasion and lymph node metastasis.

关 键 词：基质金属蛋白酶-2 喉 鳞状细胞癌 免疫组化 淋巴结转移 

分 类 号：R739.65[医药卫生—肿瘤]