NO-1886对高糖高脂喂养小型猪肾脏蛋白激酶C表达的影响  

The Effect of NO-1886 on the Expression of Protein Kinase C in the Kidneys of Diabetic Minipig Model Induced by High-sucrose and High-fat Diet

作　　者：高天舒[1] 刘毅[2,3] 李宏光[2] 祖秀宏[2] 尹卫东[2] 王宗保[4] 

机构地区：[1]南华大学医学院临床技能教学中心,衡阳421001 [2]南华大学生命科学与技术学院心血管病研究所,衡阳421001 [3]广东韶关学院医学院,512026 [4]南华大学实验动物学部

出　　处：《医学研究杂志》2008年第11期59-63,共5页Journal of Medical Research

基　　金：湖南省卫生厅科研项目(ws-c2005034);湖南省科技厅科研项目(04TH4001;05TH4002;06TH400);湖南省自然科学基金项目(05JJ20006;04JJ3058)

摘　　要：目的探讨合成化合物脂蛋白酯酶活化剂NO-1886对高糖高脂喂养小型猪肾脏蛋白激酶C表达的影响。方法广西巴马小型猪共15只,按体重随机分为3组,5只/组:正常对照组,喂正常猪饲料;高糖高脂组,喂高糖高脂饲料;加药组,喂加入1.0%NO-1886的高糖高脂饲料。动物分栏喂养,每日投食3次,日粮为体重的4%,自由饮水。实验期为5个月。实验前和实验过程中每月末分别抽取血样,葡萄糖氧化酶法检测空腹血糖;GPO-PAP酶法测定血浆三酰甘油;放射免疫法测血浆胰岛素。第5个月从左肾下极的相同部位取部分组织(包括皮质和髓质),HE染色以观察细胞显微结构,同时免疫组化染色;免疫组织化学方法和Western blot方法分别检测肾脏蛋白激酶C表达情况。结果使用NO-1886组的葡萄糖、胰岛素和三酰甘油水平显著下降;肾脏细胞显微结构结构基本正常;免疫组织化学方法和Western blot方法检测的结果同时说明NO-1886可以降低高糖高脂饮食所致巴马小型猪肾脏组织中蛋白激酶C的表达。结论NO-1886可以通过降低糖尿病肾病中的高血糖和高血脂,下调巴马小型猪肾脏组织中蛋白激酶C的表达。Objective To investigate the protective the effect of NO - 1886 on the expression of protein kinase C in the kidneys of diabetic minipig model induced by high - sucrose and high - fat diet. Methods 15 Guangzhou minipigs aged 3 months were randomly divided into 3 groups of normal control,diabetes, diabbetes treatment, which were fed by basaldiet, high sucrose and high fat feed or with 1. 0% No - 1886 respectively. These minipigs were killed at the end of 5th month. Minipigs fed with high fat/high sucrose diet were treated with No- 1886,and The fasting concentrations of plasma glucose,triglyceride,serum insulin and I：＇KC were observed. Results High fat high sucrose feeding elevated fasting plasma glucose,trglyceride and serum insulin levels significantly. Supplement of No - 1886 into high fat high sucrose diet induced a decrease in plasma glucose,triglyceride,insulin and PKC concentration compared with pigs fed with the sole high fat high sucrose diet. Conclusion No - 1886 suppressed plasma glucose,triglyceride,insulin and PKC level.

关 键 词：脂蛋白酯酶活化剂(NO-1886) 糖尿病肾病(DN) 蛋白激酶C(PKC) 高血糖 

分 类 号：R774.1[医药卫生—眼科] R587.1[医药卫生—临床医学]