乙醇辅助盐析分离猪胃蛋白酶  

Salt-out for Separating the Swine Pepsin Assisted by Ethanol

作　　者：王莹[1] 崔素萍[1] 张丽萍[1] 

机构地区：[1]黑龙江八一农垦大学食品学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2008年第5期54-56,共3页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省科技厅"十一五"重点课题(GB06B403-3)

摘　　要：为确定盐析分离猪胃蛋白酶的最佳分离工艺条件,以猪胃粘膜为原料,经盐酸浸提,通过乙醇辅助盐析对照试验、透析冷冻干燥得猪胃蛋白酶比活力为102927.5U·g-1,酶经fSephadex-G75洗脱得到单一蛋白峰,再经SDS-聚丙烯酰胺凝胶电泳,表现为单一蛋白,酶的亚基相对分子质量约为35ku。To optimize the technology of the swine pepsin separation, the swine gastric mucosa was extracted by hydrochloric acid. And the crude enzyme was purified through salting-out, dialyzing, freezing drying and sephadex G-75 gel filtration. The purified enzyme was demonstrated by SDS-PAGE to be a homogeneous protein, its molecular mass was 35 ku and the activity was 102 927.5 U·g^-1.

关 键 词：猪胃蛋白酶 分离 盐析 酶活 

分 类 号：Q556.9[生物学—生物化学]